The effect of temperature on the rate of proton transport and ATP hydrolysis by plasma membrane (PM) and tonoplast (TN) ATPases from barley (Hordeum vulgare L. cv CM 72) roots were compared. Rates of proton transport were estimated using the fluorescent amine dyes quinacrine and acridine orange. The ratio between rate of transport and ATP hydrolysis was found to depend on the dye, the temperatu...

Intrahepatic bile duct epithelial cells, or cholangiocytes, contribute to bile secretion in response to hormones, including secretin. However, the mechanism by which secretin stimulates ductular bile flow is unknown. Since recent data in nonhepatic epithelia have suggested a role for exocytosis in fluid secretion, we tested the hypothesis that secretin stimulates exocytosis by isolated cholangi...

Only a very small number of the known substances is strongly carcinogenic. This indicates that carcinogenicity is linked to different qualities which all must be present to produce cancer. The various chemical reactions or qualities brought into relation with carcinogenicity reflect one or more of these factors. None of them seems to comprise all involved parameters. At the same time, none of t...

Degradation of acridine orange (AO) in aqueous solution by anodic oxidation with a Ru-doped Pt electrode (RuDPt) can be applied to the remediation of waste waters containing acridine orange. This environmentally friendly method decontaminates completely aqueous solutions of this dye. The COD value decreases to ~98% of the initial COD. Acridine orange (AO) is more rapidly removed in RuDPt electr...

The impact of sperm DNA fragmentation on assisted reproductive technology (ART) successes, in terms of outcome, is now established. High levels of DNA strand breaks severely affect the probability of pregnancy. The importance of sperm nucleus condensation in early embryogenesis and, subsequently, on the quality of the conceptus is now emerging. In this article we have compared in situ analyses ...

Acridine derivatives constitute a class of compounds that are being intensively studied as potential anticancer drugs. Acridines are well-known for their high cytotoxic activity; however, their clinical application is limited or even excluded because of side effects. Numerous synthetic methods are focused on the preparation of target acridine skeletons or modifications of naturally occurring co...

There have been reported suitably designed, simple monoand dinuclear metal complexes which are efficient in hydrolyzing phosphate esters including DNA. We have previously reported the dinuclear Cu(II)2L1 complex (L1: 1,3-bis(1,4,7triaza-1-cyclononyl)propane) efficiently hydrolyzed DNA, producing nicked and linearized DNA over the mononuclear CuL2 complex (L2: 1,4,7-triazacyclononane). We were i...

Problem statement: Cellular drug resistance to anticancer agents is major obstacle in cancer chemotherapy and the mechanisms by which these MDR cells possess for protecting themselves to survive prolonged exposure to cytotoxic agents still debating. The study aimed to clarify the role of P-glycoprotein (Pgp) and enhanced drug sequestration in lysosomes to confer the multidrug resistance K562 ce...

Benz[a]acridine was considered by previous IARC Working Groups in 1983 and 1987 (IARC, 1983, 1987). Since that time, new data have become available; these have been incorporated into the Monograph, and taken into consideration in the present evaluation. Benz[c]acridine was considered by previous Working Groups in 1972, 1983, and 1987 (IARC, 1973a, 1983, 1987). Since that time, new data have bec...

Two recently synthesized groups of acridine cytostatics containing fused heterocyclic ring(s): pyrazoloacridines (PAC) and pyrazolopyrimidoacridines (PPAC) were tested in regard to their in vitro cytotoxic activity towards a panel of sensitive and resistant human tumor cell lines. The obtained results corroborate our earlier hypothesis on the essential role of heterocyclic ring fused to the acr...