Barrett's oesophagus is an acquired precancerous condition that develops from mucosal injury incurred due to chronic gastro-oesophageal reflux. The aim of this study was to determine if bile and/or acid components of the refluxate can induce DNA damage in vitro. The oesophageal cell lines FLO-1 and HET1-A were exposed to primary bile salts, individually or as a mixture, and the secondary bile s...

Chloroperoxidase and horseradish peroxidase use NaClOz as both the oxidant and the halogen donor for the peroxidative chlorination of monochlorodimedone. Previous studies have shown that both horseradish peroxidase and chloroperoxidase can catalyze iodination reactions with hydrogen peroxide as the oxidant; however, only chloroperoxidase catalyzes chlorination reactions under these conditions. ...

Horseradish peroxidase and chlorite, NaC102, are able to catalyze chlorination of monochlorodimedone to form dichlorodimedone. Catalytic amounts of horseradish peroxidase act to disproportionate chlorite forming chlorine dioxide and chloride ion. The chlorine dioxide thus formed is responsible for the chlorination of monochlorodimedone. It was previously thought (Chiang, R., Rand-Meir, T., Maki...

* The trichloroacetic acid extracts were first acidified with sulfuric acid3 and kept overnight at 38” for decarboxylation of acetoacetate. The fluid was then neutralized with sodium hydroxide and phosphate, and the acetone distilled by the procedure of Folin.4 Acetone was determined calorimetrically by the method of Behre.6 Analogous results were obtained with the determination procedure of Le...

Two methods using nujol and hexachloro-butadiene mulls are described to analyse the water content of powdered granite samples. The nujol mull analyses were carried out on a Unicam SP Ioo spectrometer using the silica content of the rock as an internal standard. The hexachlorobutadiene mull analyses were done by a twin-path Unicam SP 2oo spectrometer, using hexachlorobutadiene as the internal st...