The phenotypic and quantitative relationship of red cell acid phosphatase with haemoglobin, haptoglobin, and G6PD phenotypes was investigated in three populations in the Sudan and one population in Nilgiris, India. No significant consistent association of red cell acid phosphatase phenotypes was observed with these polymorphisms. However, there was a lack of acid phosphatase AB in G6PD deficien...

PURPOSE To determine whether soluble CD44 (sCD44), a likely biomarker of primary open-angle glaucoma (POAG), is internalized in cultured human trabecular meshwork (TM) cells and trafficked to mitochondria. METHODS In vitro, 32-kD sCD44 was isolated from human sera, biotinylated, and dephosphorylated. TM cells were incubated for 1 hour at 4°C with biotinylated albumin (b-albumin), biotin-label...

In the current opinion, we would like to study adhesion of DNA and RNA−amphiphile complexes to non–modified glasses, glasses containing phenylboronic acid (PBA) attached via 3– glycidoxypropyltrimethoxysilane (GPTMS) [(PBA–GPTMS–glasses)] and glasses containing grafted dimethyllacrylamide–acrylamide phenylboronic acid (DMAA–AAPBA) copolymer (DMAA–AAPBA– glasses). We prepared glasses modified wi...

Ouabain-sensitive K influx into ground squirrel and guinea pig red cells was measured at 5 and 37 degrees C as a function of external K and internal Na. In both species the external K affinity increases on cooling, being three- and fivefold higher in guinea pig and ground squirrel, respectively, at 5 than at 37 degrees C. Internal Na affinity also increased on cooling, by about the same extent....

Chloramphenicol (D-threo-2-dichloroacetamido-1-p-nitrophenylpropane-1,3-diol) added to blood samples did not alter the observed shift in the red cell osmotic fragility curves as the samples were aged in vitro for 24 hours at 37 degrees C. Nor was the normal rate of loss of 2,3-diphosphoglycerate, ATP, or glutathione from the red cells affected by the presence of chloramphenicol over the same pe...

Human red blood cells reach an equilibrium of phosphate distribution at 37 ° much faster with o . I I M or intermediate levels than with o.o2 M and lower phosphate levels. Other divalent anions added to 0.o2 M phosphate solutions have an action similar to that of the extra phosphate. A past observation that phosphate escapes from red cells more slowly than it enters them appears to have arisen ...

Ca2(+)-ATPase activity in human red cell membranes is dependent on the presence of calmodulin. All trans-retinoic acid inhibited human red cell membrane Ca2(+)-ATPase activity in vitro in a concentration-dependent manner (10(-8) to 10(-4) M). In contrast, retinol, retinal, 13-cis-retinoic acid and the benzene ring analogue of retinoic acid did not alter enzyme activity. Purified calmodulin (up ...

The routine pretransfusion red blood cell antibody detection test (PADT), performed at the authors' institution, consists of a threephase, saline-antiglobulin technique (immediate spin, 30-minute incubation at 37 °C, IgG indirect antihuman globulin test |IAT|), and each phase is examined for hemolysis and agglutination. To determine if it would be safe to omit reading the 37 °C phase of the PAD...