Previous studies of the choline acetylation system of brain and of the head ganglion of the squid have utilized acetate, ATP,’ and CoA to generate “active acetate” (1, 2). Following the isolation of acetyl CoA by Lynen et al. (3), its identity with “active acetate” has been established by the demonstration that the acetylated coenzyme is the acetyl donor in the biological acetylation of sulfani...

Acetyl-CoA produced in mitochondria from carbohydrate or amino acid catabolism needs to reach the cytosol to initiate de novo synthesis of fatty acids. All eukaryotes analyzed so far use a citrate/malate shuttle to transfer acetyl group equivalents from the mitochondrial matrix to the cytosol. Here we investigate how this acetyl group transfer occurs in the procyclic life cycle stage of Trypano...

The front cover picture was designed by C. Zippel, Z. Hassan and S. Bräse. Kinetic resolution of 4-acetyl[2.2] paracyclophane via ruthenium-catalyzed enantioselective hydrogenation has been realized. This method can be performed on a multigram-scale gives access to enantiomerically pure derivatives with planar central chirality (Rp)-4-acetyl-PCP (97% ee, 43%) (Sp,S)-PCP 46%), which are useful i...

The presence of the acetyl low density lipoprotein (acetyl-LDL), or scavenger receptor, which binds modified forms of LDL, was examined in rat luteal cells. Acetyl-LDL supported progesterone production by dispersed rat luteal cells at least equal to that of the native LDL under basal conditions and in the presence of human chorionic gonadotropin (hCG). The acetyl-LDL-supported progesterone prod...

Usingdifferent conditions for incubation and fluorometry with 4-methylumbelliferylglycosides as substrates, we demonstratedthe presence ofacida-glucosidase, “renal” a-glucosidase, N-acetyl--D-glucosaminidase A, and N-acetyl-f3-o-glucosaminidase B in freshly drawn normal human serum. The acid a-glucosidase enzymatic activity was determined at pH 4.0 in 0.1 mol/L Tris reagent, whereasthe renal is...

A new enzyme, acetate:SH-[acyl-carrier-protein] enzyme ligase (AMP), has been purified about 30-fold from cell-free extracts of Klebsiella aerogenes. The enzyme, in the presence of acetate and ATP, catalyzes the acetylation of enzymically inactive des-acetyl citrate lyase to enzymically active citrate lyase (EC 4.1.3.6). Acetate and ATP could be replaced by acetyl adenylate. Acetyl-CoA can act ...

An O-acetyl group was selectively introduced into the ceramide moiety at the C-3-O on ganglioside GM3 containing N-acetyl neuraminic acid, the product of which has been previously found in rat glioma tissue as a glioma-associated ganglioside. The introduction of the acetyl residue involved a two-step process involving per O-acetylation of GM3 and saponification with a mild alkaline solution in ...

Acetyl coenzyme A (acetyl-CoA) is a central metabolite in carbon and energy metabolism. Because of its amphiphilic nature and bulkiness, acetyl-CoA cannot readily traverse biological membranes. In fungi, two systems for acetyl unit transport have been identified: a shuttle dependent on the carrier carnitine and a (peroxisomal) citrate synthase-dependent pathway. In the carnitine-dependent pathw...

Indole-3-acetyl-myo-inositol galactoside labeled with 3H in the indole and 14C in the galactose moieties was applied to kernels of 5 day old germinating seedlings of Zea mays. Indole-3-acetyl-myo-inositol galactoside was not transported into either the shoot or root tissue as the intact molecule but was instead hydrolyzed to yield [3H]indole-3-acetyl-myo-inositol and [3H]indole-3-acetic acid w...