Based on IR absorbance spectra of healthy and malignant breast [1] and blood cells [2] found in literature we investigated the possibility of disease stage cell discrimination by comparing only a few absorbance peaks. This way no expensive FTIR spectroscope or time consuming expensive staining/labeling steps are required. Absorbance differences between healthy and malignant cells at wavelengths...

A simple and economical dual wavelength spectrophotometric method has been developed for the simultaneous estimation of atorvastatin calcium and amlodipine besylate in their combined dosage forms. The method was based on property of additivity of absorbances. The two wavelengths on amlodipine besylate curve were found out where it showed same absorbance, which were 257.4 and 360.0 nm. At 360.0 ...

This work discusses regular particle arrays as nanostructured front layers for possible application in photovoltaic devices yielding strongly increased forward scattering. I used a rigorous plane-wave method to investigate multi-type particle layers combining different radii and configurations. The absorbance was enhanced compared to the bare Si wafer and I demonstrated on mixing particles a br...

We report a new kinetic approach to the quantification of triglycerides (triacylglycerols) in human sera. The new approach combines a commercially available enzyme-reagent system with a multiple-linear-regression data-processing method. Values of absorbance collected at 3-s intervals between 20 and 305 s are fitted to a pseudo-first-order model to compute the total change in absorbance expected...

In this study, zero-, first-, secondand third-order derivative spectrophotometry methods were developed for the determination of metoprolol in pharmaceutical preparations. In zero order spectrophotometry, absorbance values were measured at 276 nm in zero order spectra of solution of metoprolol in methanol in the range of 240-310 nm. In first derivative spectrophotometry, absorbance values were ...

In HPLC, the detector is selected in accordance with the analyte. Many of the detectors used are based on optical absorbance, such as UV-Vis absorbance detectors and photodiode array (PDA) detectors. Absorbance detectors have the following features: The detector sensitivity and selectivity can be adjusted by setting an appropriate detector wavelength. The sensitivity is relatively high (althoug...

We describe a new technique for monitoring intra-cuvette temperatures in temperature-controlled spectrophotometric cells by use of two solutions, one for which absorbance is highly sensitive to temperature and one for which absorbance is insensitive to temperature. The first solution, cresol red in tris(hydroxymethyl)aminomethane buffer, is used to construct an absorbance-vs.-temperature calibr...

This report discusses characteristics of a custom-designed vidicon spectrometer and evaluates its applicability to several clinical analysis problems. Data show that the vidicon detector response is linear with intensity over about four orders of magnitude and that the uncertainty in absorbance measurements can approach 0.001 absorbance units in the range from 0 to 2 absorbance units. Applicati...

Light-induced absorbance changes in the near-infrared (NIR) spectral region (700 -950 nm) are analyzed in isolated spinach chloroplasts using pulsed light-emitting diodes as modulated measuring light sources. With chloroplasts under coupled conditions the light-induced signal is dominated by a relatively slow scattering change displaying a flat difference spectrum. More specific changes can be ...