نتایج جستجو برای: a 355 bp fragment was amplified from s
تعداد نتایج: 14809709 فیلتر نتایج به سال:
We purified a 29-kDa Helicobacter pylori outer membrane protein (Omp29 protein) and cloned the gene encoding the protein from H. pylori strain ATCC 43504. The Omp29 gene corresponded to the reported JHP73 and the HP78-79 genes of H. pylori strains. A corresponding nucleotide fragment was detected in all 150 tested H. pylori clinical isolates by PCR or Southern blotting. The amplified Omp29-corr...
Sets of oligonucleotide primers were designed according to the sequences of the open reading frames (ORFs) ORF1 and ORF2 of the prototype nonpathogenic PK-15 strain of porcine circovirus (PCV) type 1 (PCV-1). By the PCR performed with the various primer sets, genomic DNA or RNA from other bacterial or viral pathogens of the respiratory tracts of pigs could not be amplified. A positive amplifica...
toury (1978:200) believes that translation is a kind of activity which inevitably involves at least two languages and two cultural traditions. being polite while asking for something takes place differently in different cultures and languages, therefore various strategies may be applied for making requests and also translation of them in order not to disturb or threaten the face or better to sa...
This study was carried out to identify polymorphisms of BMP15, GDF9 and BMPRIB genes in cross- bred of Romanov×shal sheep. For this reason 79 blood samples were collected from a flock in Gonbad kavous city in Golestan province. DNA was extracted using modified salting out method. Polymerase chain reaction was done by specific primers for amplification of two fragments with 141and 153 bp from ...
Toxoplasma gondii contain various immunogenic antigens. The most important Toxoplasma antigens are somatic and excreted/secreted antigens. Rhoptry proteins are known as excreted/secreted antigens. These antigens have been proposed as a vaccine candidate against toxoplasmosis. The main objective of the present work was cloning rhoptry protein1 (ROP1) Gene of Toxoplasma gondii (RH) in a cloning...
esl/efl books play a crucial role in shaping language learners worldview of gender roles in society. the present study investigated the status of sexism in two sets of efl textbooks, one developed by non-native iranian authors (ili series) and the other by native authors (top notch series). first, two books from each series was selected randomly. then, a quantitative analysis was carried out wi...
the issue of curriculum and syllabus evaluation and revision has been in center of attention right from when curriculum came into attention of educational institutions. thus everywhere in the world in educational institutions curricula and syllabi are evaluated and revised based on the goals, the needs, existing content, etc.. in iran any curriculum is designed in a committee of specialists and...
this study was an attempt to investigate the effect of teaching meta cognitive strategies on iranian intermediate efl students speaking proficiency. in this study the researcher has employed metacognitive strategies taken from brown (2000) to teach speaking strategies to number of participants. the participants were intermediate students of shokouh language institute; they were divided into an ...
A nested PCR method was developed for the detection of Coxiella burnetii in human serum samples. Two pairs of oligonucleotide primers were designed to amplify a 438-bp fragment of the com1 gene encoding a 27-kDa outer membrane protein of C. burnetii. The primers amplified the predicted fragments of 21 various strains of C. burnetii but did not react with DNA samples from other microorganisms. T...
Human genes encoding tumor necrosis factor (TNF-a) and lymphotoxin (TNF-/3) are tandemly arranged (1) and located at 6p21.3 (2, 3). Their precise position within MHC has been determined (4, 5). We recently sequenced a 820 bp fragment located 3.5 kb upstream to TNF genes centromeric to HLA-B and used the sequence surrounding the two linked microsatellites to study TNF gene polymorphism (6). We n...
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