Unconjugated bilirubin (UCB) is almost insoluble in water at neutral pH, but appears in normal human gallbladder bile at concentrations up to 35 microM. We therefore determined whether conjugated bile salts could increase the dissolved concentration [( Bt]) of UCB over the pH range 3.0-11.0. Using crystalline UCB, [Bt] was higher with less ordered crystals, with increasing pH and bile salt conc...

identification and use of more efficient enzymes in the food and pharmaceutical industries is the focus of many researchers. the aim of this study was to search for a new bacterial strain capable of producing high levels of pullulanase applicable to biotechnology, the starch bioprocessing and food industries. a new pullulan hydrolyzing bacillus strain was isolated and designated sdk2. morpholog...

The production of extracellular amylase by the halotolerant fungus Penicillium sp. was statistically optimized in a submerged cultivation. Maximum enzyme production was obtained after two days of incubation in a medium containing 10% (w/v) NaCl, in addition to MgSO .7H O 1% and 1% (w/v) of 4 2 starch. The optimum pH for growth and enzyme production was 4. Partial characterization of the crude e...

In environments with temperatures above 60 degrees C, thermophilic prokaryotes are the only metabolically active life-forms. By using the SO(4) tracer technique, we studied the activity of sulfate-reducing microorganisms (SRM) in hot sediment from a hydrothermal vent site in the northern part of freshwater Lake Tanganyika (East Africa). Incubation of slurry samples at 8 to 90 degrees C demonstr...

Polyphenol oxidase from Black mulberrys was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. P-cresol was the better substrate for cresolase activity with a Km (11.5 mM) and catechol for catecholase activity with Km (6.4 mM) at pH 5. Km for cresolase and catecholase activity of IsoPPOII at pH 6.5 were 12 and 8.5 and for IsoPPOIII at pH ...

In order to estimate responses of growth rate and growth hormone of Clarias gariepinus to stocking density, fish (initial weight 30.71 ± 0.89 g) were stocked in 120-L tanks at densities of 35, 65, 95, and 125 kg/m3 for 60 days. On day 30 and 60, the fish growth rates were measured, pituitary growth hormone (GH) mRNA levels and serum GH concentrations were examined by real-time qPCR and ELISA te...

objective: investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (smf). methods: optimum temperature for enzyme activity in the crude extract was 40 ◦c at a ph between 8.0 and 9.0. the studies on ph stability showed that the enzyme was stab...