Delayed Maturity of Bean Plants Sprayed With Solutions of 2,4-Dichlorophenoxyacetic Acid of Nonherbicidal Concentrations: E. R. Stromme and C. L. Hamner ........................ 170 Survival Time of Various Warm-blooded Animals in Extreme Cold: S. M. Horvath, et al. 171 The Respiration of Streptomyces griseus: David Gottlieb and H. W. Anderson .................. 172 Sucrose in the Stalks of Mai...

An inducible, cytosolic glutathione S-transferase (GST) was purified from Streptomyces griseus. GST isoenzymes with pI values of 6.8 and 7.9 used standard GST substrates including 1-chloro-2,4-dinitrobenzene. GST had subunit and native M(r)s of 24 and 48, respectively, and the N-terminal sequence SMILXYWDIIRGLPAH.

During 2 years, from March 1987 to April 1989, 600 samples of soil and plant were collected from different cities of northern provinces of Iran. Out of the six hundred samples 58 Actinomycetes, 9 Pseudallescheria boydii, 10 Sporothrix schenckii, and 4 Sporothrix schenckii-like fungi were isolated as the following: Streptomyces griseus from 31 samples (38.27%), Nocardia asteroides from 8 sa...

The new antibiotics 4181-A and B were isolated from the fermentation broth of Streptomyces griseus, a soil isolate. Their molecular formulae were determined as C29H21NO9 and C28H19NO9, respectively. The UV, IR and NMR spectra suggest that they possess a quinone moiety in their structures. They were found to have antibacterial, antifungal and antitumor activity.

39 Applying in silico simulations and in vitro experiments, the amino acid proline was proved to 40 have a profound influence on Streptomyces griseus trypsinogen, and the hydrogen bond 41 between H and D was crucial for trypsin activity. By introducing an artificial propeptide 42 IVEF, the titer of trypsin was increased by 6.71-fold. 43

An earlier paper (Lechevalier, Acker, Corke, Haenseler, and Waksman, 1953), announcing the addition of candicidin to a rapidly growing list of antifungal antibiotics, described the morphological and cultural characteristics of Streptomyces griseus 3570, from which candicidin originated, its selective antibiotic action, and methods by which the agent itself could be isolated from culture broths ...

The streptomycin-producing capacity of submerged cultures of Streptomyces griseus 107 was nearly lost during repeated transfers on synthetic nutrient medium. This effect did not occur on the same nutrient medium from which Fe(++) was omitted. The physiological reason for this effect was discussed from the point of the relationship of iron-containing porphyrin enzymes to the process of streptomy...

Within recent years, phages capable of infecting various Streptomyces species have been reported (Saudek and Colingsworth, 1947; Reilly et al., 1947; Koerber et al., 1950; Perlman et al., 1951; and Alexander and McCoy, 1956). Hoehn (1949) and subsequently Gilmour and Buthala (1950) demonstrated that streptomyces phage is indigenous to soil, the natural habitat of the host. The streptomyces host...

The nucleotide sequence of the Streptomyces griseus afsA gene, possibly encoding a key enzyme for A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) biosynthesis, was determined. The translational initiation codon was identified by introducing out-of-frame mutations at appropriate positions by oligonucleotide-directed mutagenesis. The afsA gene was thus found to code for a protein o...