We have studied maturational changes in the kinetics of the proximal tubule Na+/H+ antiporter. Microvillus membrane vesicles were prepared from renal cortex of fetal and newborn lambs. Amiloride-sensitive uptake of 22Na+ by these vesicles was measured and Woolf-Augustinsson-Hofstee plots were used to determine the Michaelis constant (Km) and rate of maximal uptake (Vmax). Initial studies of fet...

Removal of the mRNA 5' cap is an important step in the regulation of mRNA stability. mRNAs are degraded by at least two distinct exonucleolytic decay pathways, one from the 5' end, and the second from the 3' end. Two major cellular decapping enzymes have been identified, and each primarily functions in one of the two decay pathways. The Dcp2 decapping enzyme utilizes capped mRNA as substrate an...

Gravitational lens time delays depend on the Hubble constant, the observed image positions, and the surface mass density of the lens in the annulus between the images. Simple time delay lenses like PG1115+080, SBS1520+530, B1600+434, PKS1830–211 and HE2149–2745 have H 0 = A(1 − κ) + Bκ(η − 1) where the two coefficients A ≃ 90 km/s Mpc and B ≃ 10 km/s Mpc depend on the measured delays and the ob...

Amputee patients spend more energy during walking than able-bodied persons. It is generally accepted that more proximal amputation is associated with more energy need. However, the picture is not clear, especially for partial foot amputees. The purpose of this study was to compare the energy consumption of walking in traumatic unilateral amputees with three different amputation levels (trans-fe...

two different growth media, namely complex and defined media, were used to examine establishment of steady-state conditions in phosphate-limited culture system of saccharomyces cerevisiae cen.pk113-5d strain. using the defined growth medium, it was possible to obtain steady state condition in the continuous culture. the effect of phosphate concentration on the growth of s. cerevisiae in phospha...

Background. Evidence suggests that improved locational access to farmers' markets increases fruit and vegetable (FV) consumption, particularly for low-income groups. Therefore, we modelled potential alternative distributions of farmers' markets in one country (New Zealand) to explore the potential impact for deprived populations and an indigenous population (Māori). Methods. Data were collected...