Shuttle vectors that replicate stably and express selectable phenotypes in both Thermococcus kodakaraensis and Escherichia coli have been constructed. Plasmid pTN1 from Thermococcus nautilis was ligated to the commercial vector pCR2.1-TOPO, and selectable markers were added so that T. kodakaraensis transformants could be selected by DeltatrpE complementation and/or mevinolin resistance. Based o...

CONTEXT Polycystic ovary syndrome (PCOS) is a triad of anovulation, insulin resistance, and hyperandrogenism. Androgen excess may correlate with metabolic risk and PCOS consensus criteria define androgen excess on the basis of serum T. Here we studied the utility of the androgen precursor serum androstenedione (A) in conjunction with serum T for predicting metabolic dysfunction in PCOS. PATIE...

The present study in the wall lizard, Hemidactylus flaviviridis, was aimed to understand the role of histamine (HA) in the regulation of Leydig cell and testicular macrophage activities, for the first time, in ectothermic vertebrates. Although HA did not affect the testosterone production from unstimulated Leydig cells, it had dual concentration-related effects, stimulatory at a low concentrati...

The polymorphic minor histocompatibility Ag HA-1 locus encodes two peptides, HA-1(H) and HA-1(R), with a single amino acid difference. Whereas the immunogenicity of the HA-1(R) allele has not yet been shown, the nonameric HA-1(H) peptide induces HLA-A2-restricted cytotoxic T cells in vivo and in vitro. It is not known whether the mHag HA-1(H) or HA-1(R) associates with other HLA class I molecul...

A field experiment was conducted at the Soil Science Field Laboratory of Bangladesh Agricultural University (BAU), Mymensingh during Aman season of 2011 to evaluate the effects of manures and fertilizers for maximizing the yield of BRRI dhan49. The experiment had six treatments, laid out in a randomized complete block design (RCBD) with four replications. The treatments were T0 = Control, T1 = ...

Adherence to hydroxyapatite (HA) was examined as a method to concentrate bacteria from foods. Using HA at a level of 10% and suspensions of an Escherichia coli strain containing 10(9), 10(6), and 10(3) cells per ml, kinetic studies revealed that maximum adherence was attained within 5 min for all cell concentrations and that comparable log reductions (1.0 to 1.5) of cells in suspension were see...

Influenza epidemics occur annually, and estimated 5-10% of the adult population and 20-30% of children will become ill from influenza infection. Seasonal vaccines primarily work through the induction of neutralizing antibodies against the principal surface antigen hemagglutinin (HA). This important role of HA-specific antibodies explains why previous pandemics have emerged when new HAs have app...

A pronounced residual effect of organic and inorganic fertilizers, applied in maize crop was observed on yields of maize-gobhi sarson cropping system. The experiment was conducted with 10 treatments of N, P, K, FYM, crop residue and zinc sulphate nutrients. The highest growth, yield and yield components of maize crop were recorded with 100% recommended fertilizer dose-RFD + ZnSO4 20 kg/ha and t...

Low soil fertility and high weed infestation are the main culprits for the declining maize production inWestern Kenya. Technology packages to address these constraints exist, but their effectiveness is likely to be influenced by variability in soil types and farm management practices in the region. Trials were conducted during the 2008/2009 cropping seasons to investigate the nutrient use e...

In the extreme climatic conditions of steppe area Saratov region, one primary factors in improving alfalfa productivity are new highly productive and adaptive varieties. The purpose current study was to identify most promising populations according feed for development varieties Trans-Volga region. zoned blue variety ‘Yuzen’ used as standard variety. included State List 2006 Middle Volga Lower ...