To investigate whether primary cultures of adult rat hepatocytes retain the capacity to respond to inducers of cytochrome P-450 other than polycyclic aromatic compounds, we tested the effects of pregnenolone-16acarbonitrile (PCN) which induces a novel form of the hemoprotein (PCN cytochrome P-450) i n rat liver (Elshourbagy, N. A., and Guzelian, P. S. (1980) J. EioL Chem, 255,1279-1286). Cultur...

The hydroxylating activities of liver microsomes for proges terone and testosterone were markedly decreased in male and female rats bearing Walker carcinosarcoma 256. The decreases in the hydroxylating activities were greater in the male rats than in the female rats. The content of cytochrome P-450 in liver microsomes was decreased in the tumor-bearing male and female rats. Moreover, the bindin...

A cytochrome P-450 from neonatal pig testicular microsomes was purified to homogeneity as judged by electrophoresis on sodium dodecyl sulfate-polyacrylamide gels and by double diffusion on agar against antiserum raised in rabbits against the protein. The enzyme shows both 17 alpha-hydroxylase (Vmax = 4.6 nmol of product/min/nmol of P-450, Km = 1.5 microM) and C17,20 lyase (Vmax = 2.6 nmol of pr...

Cytochrome P-450 (P-450) content and laurate-omega-oxidation activity in rat kidney and liver microsomes were investigated following starvation. Multiple forms of P-450 were analyzed by one dimensional separation using peroxidase stained SDS-continuous gradient polyacrylamide gel electrophoresis. Gels of the hepatic microsomes treated with phenobarbital showed three P-450 bands, and the renal m...