We report a novel method of electrochemical signaling from antigen-antibody interactions at immunoelectrodes with bioelectrocatalyzed enzymatic signal amplification. For the immunosensing surface construction, a poly(amidoamine) G4-dendrimer was employed not only as a building block for the electrode surface modification but also as a matrix for ligand functionalization. As a model biorecogniti...

Mice immunized with hapten-autologous serum albumin conjugates (DNP-mouse serum albumin) were shown to contain immune B and T cells with specificity for the conjugate. Fractionation on antigen-coated Sepharose beads showed that B cells could be subdivided in two major groups: those reacting against the haptenic group (DNP) and those reactive against the new antigenic determinants (NADs) introdu...

A hapten (DNP) model of topically induced ocular anaphylaxis has been developed. Rats immunized with DNP-Ascaris were skin-tested with DNP-bovine serum albumin (DNP-BSA) and Evans blue and challenged topically with varying amounts of di-DNP-lysine. The degree of clinical conjunctival edema was assessed, and eye tissues were evaluated histologically. Clinical conjunctival edema and histologic ma...

The (B-cell) repertoire responsive to the DNP and TNP haptenic determinants in BALB/c neonates was analyzed in terms of the specificity of stimulation of neonatal B cells as well as the diversity of specificities available in neonatal populations. The results indicate that the parameters of stimulation of neonatal B cells are similar to those of nonimmune adults, particularly in the exquisitely...

The metabolism of antibody-excess immune complexes was compared in lactating and normal female BALB/c mice. The initial rate of clearance of the antigen, dinitrophenyl- (DNP) ovalbumin, when complexed with mouse anti-DNP antibody was considerably faster in lactating mice. The half-life was 4 min in lactating mice compared with 8 min in normal female mice. Tissue distribution studies revealed th...

This case study reflects the journey taken by multiple individuals as they considered doctor of nursing practice (DNP) evolution within one suburban acute care setting. The DNP education that chief officer (CNO) experienced led to a major change for all leaders in organization. Two CNOs provide their unique path and insights building leadership team composed primarily DNP-prepared leaders, sust...

after years of trying to make independent phd course, nursing could create two independent course including research-focused course (phd) and clinical practice -focused (dnp). phd focused more on research and dnp emphasis more on clinical activities and innovative clinical application of evidence-based research results. presence or absence of dnp is still a debate as a challenge in nursing. in ...

An appropriate concentration of DNP (2, 4-dinitrophenol) is capable of inhibiting the ATP energized mitochondrial volume change induced by gramicidin in the presence of the permeant ions potassium and L-malate. The further addition of the non-mercurial thiol reagent, showdomycin reinstates, an effect of gramicidin which is dependent upon potassium ion. Thus the strategically located nucleophili...

We have investigated the dynamic nuclear polarisation (DNP) of 298i nuclei in highly doped silicon single crystals. The saturation of the electronic Zeeman levels in a magnetic field of 3.3 kG at 1.60)( was obtained using microwave ultrasonics of about 9 GHz. The ultrasonic waves were generated in a cavity by means of a transducer consisting of a thin film of CdS evaporated on the polished surf...

The mechanism of selective anti-hapten IgE antibody production was studied in SJL mice. Using an adoptive transfer method of spleen cells into syngeneic recipients irradiated with a sublethal dose of 600 rads, it was demonstrated that for the suppression of anti-dinitrophenyl (DNP) IgE antibody production the interaction of two subsets of T cells is necessary. DNP-primed B cells and carrier-pri...