DRUGS AND UPTAKE OF 32p BY ERYTHROCYTES 533' 5. Sodium salicylate and sodium thiopentone depressed the 32p uptake of 2:3-diphosphoglycerate and adenosine triphosphate to a greater extent than they depressed the 32P exchange of inorganic phosphate and hexose monophosphate. These agents do not depress 32p uptake by inhibiting triose phosphate dehydrogenase, for neither drug had any effect on glyc...

The dominant insulin-stimulated ribosomal protein S6 kinase activity was purified to near homogeneity from insulin-treated 32P-labeled rat H4 hepatoma cells and found to copurify with a 70-kDa 32P-labeled polypeptide. The dominant S6 kinase purified from livers of cycloheximide-treated rats is also a 70-kDa polypeptide. Antiserum raised against rat liver S6 kinase specifically immunoprecipitate...

It was observed that rat liver cytosol can transfer 32P from 32PO4 to acceptor proteins. 32P-labeled proteins co-purify with a class of proteins known to accept arginine at their NH2-terminal end. When arginine-14C is added to the incubation mixture, 32P incorporation is stimulated and small molecules bearing both 32P and 14C labels can be released by treating labeled proteins with leucine amin...

The photoaffinity label 8-azido[32P]adenosine 3':5'-monophosphate (8-azido-cyclic [32P]AMP) was used to analyze both the cAMP-binding component of the purified cAMP-dependent protein kinase, and the cAMP-binding proteins present in crude tissue extracts of bovine cardiac muscle. 8-Azido-cyclic [32P]AMP reacted specifically and in stoichiometric amounts with the cAMP-binding proteins of bovine c...

PURPOSE To determine the optimal location of catheters in multichannel vaginal applicators to appropriately cover the vaginal cuff target and minimize dose to the organs at risk (OARs) and hot spots in the target. METHODS A new multichannel vaginal applicator with a diameter of 30 mmconsists of a single central catheter and an outer array of eight catheters. A total of 20 plans were generated...

BACKGROUND AND OBJECTIVES A sharply contoured cryolipolysis vacuum applicator was developed to improve fit and tissue draw in the abdomen and flanks to better accommodate a range of body types and a variety of treatment sites. This study was carried out to evaluate the safety and efficacy of the new applicator for treatment of flank fat ("love handles"). STUDY DESIGN/MATERIALS AND METHODS A c...

The lateral buildup ratio (LBR) used to estimate the depth dose distribution of electron beams for an irregular cutout field was obtained for a 4-MeV energy beam from a Varian 21 EX linear accelerator. The depth-dose curves for a group of circular cutout fields starting from a 2-cm diameter were measured. Electron diodes were used in a large water tank to measure the LBR values for 6, 9, 12, an...

The human asialoglycoprotein receptor was isolated via immune precipitation from hepatoma Hep G2 cells following incubation with [32P]Pi. Analysis on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis revealed incorporation of 32P into both the 46 000 Da mature form of the receptor as well as the 40 000 Da precursor. The incorporated 32P was associated with phosphoserine. The degree of ...