A variety of stable isotope labeling techniques have been developed and used in mass spectrometry (MS)-based proteomics, primarily for relative quantitation of changes in protein abundances between two compared samples, but also for qualitative characterization of differentially labeled proteomes. Differential O/O coding relies on the O exchange that takes place at the C-terminal carboxyl group...

Science results from the Genesis Mission illustrate the major advantages of sample return missions. (i) Important results not otherwise obtainable except by analysis in terrestrial laboratories: the isotopic compositions of O, N, and noble gases differ in the Sun from other inner solar system objects. The N isotopic composition is the same as that of Jupiter. Genesis has resolved discrepancies ...

Topological defects, such as point defects, dislocations and grain boundaries, have a dramatic influence on the chemical and physical properties of large-scale graphene grown by chemical vapor deposition (CVD) method. Here we demonstrate the Raman visualization of polycrystalline structures in an isotopically modified CVD graphene. By means of the reversible reaction of methane on a copper cata...

Nucleolar 28 S, 35 S, and 45 S RNA's were isolated from normal rat liver, Novikoff hepatoma ascites cells, and Morris hepatoma 9618A after short (60 min) and long (6 hr) labeling with orthophosphate-32p. After purification by sucrose density sedimentation, the 32p nucleotide composition of these RNA's was determined. The RNA was hydrolyzed to oligonucleotides with pancreatic ribonuclease. The d...

In a condensation between [3-3H3]pyruvate and D-glyceraldehyde-3-P as catalyzed by 2-keto-3-deoxygluconate-6-P aldolase (EC 4.1.2.14) of Pseudomonas putida, C--C synthesis occurred appreciably faster than C--3H bond breaking. Since tritium is present in tritiated pyruvate in tracer amounts, this result showed hydrogen isotope discrimination in pyruvate deprotonation and suggests enolpyruvate ge...

Abbreviations: ABC: Ammonium Bicarbonate; AQUA: Absolute Quantification; CV: Coefficient of Variation; DOC: Deoxycholate; DTT: Dithiothreitol; Gdn: Guanidine; IS: Internal Standard; MRM: Multiple Reaction Monitoring; OATP: Organic Anion-Transporting Polypeptide; PMAX: ProteaseMAXTM Surfactant; S.E.M: Standard Error of the Mean; SIL: Stable Isotope Label; SILAC: Stable Isotope Labeling by Amino ...

We present ab-initio calculations of secondary isotope effects on NMR chemical shieldings. The change of the NMR chemical shift of a certain nucleus that is observed if another nucleus is replaced by a different isotope can be calculated by computing vibrational corrections on the NMR parameters using electronic structure methods. We demonstrate that the accuracy of the computational results is...

Monitoring of stable water isotopes (18O and 2H) in precipitation and surface waters in the Mackenzie River basin of northern Canada has created new opportunities for researchers to study the complex hydrology and hydroclimatology of this remote region. A number of prior studies have used stable isotope data to investigate aspects of the hydrological regime of the wetland-dominated terrain near...

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We present a theoretical study of the possibility to use isotope label two-dimensional infrared (2DIR) spectroscopy to obtain site specific structural information in trpzip2. This small beta-hairpin peptide was designed as a model system for studying protein folding in beta-sheet structures. In order to unravel the folding mechanism, the surroundings of local sites should be characterized, whic...