BACKGROUND Systemic lupus erythematosus (SLE), is a heterogeneous disease which predominantly affects young females (90%). SLE is associated with a shorter life expectancy than in the general population. Standardized mortality ratios (SMR) of 2.4 have been reported, which is comparable to diabetes. In modern societies cardiovascular disease (CVD) is the major cause of premature mortality. Accel...

Benzoylformate decarboxylase from Pseudomonas putida (PpBFDC) is a thiamin diphosphate-dependent enzyme that carries out the nonoxidative decarboxylation of aromatic 2-keto acids. The x-ray structure of PpBFDC suggested that Ser-26, His-70, and His-281 would play important roles in its catalytic mechanism, and the S26A, H70A, and H281A variants all exhibited greatly impaired catalytic activity....

The receptor occupancy required to produce cannabinoid effects in the central nervous system was determined in both a neurochemical and a behavioral assay for cannabinoid actions. In the neurochemical experiments, performed on superfused rat hippocampal slices, electrically evoked [3H]acetylcholine release was inhibited by the cannabinoid agonist, WIN 55212 to 2 with an EC50 of 0.005 microM and...

The third extracellular loop of the human delta-opioid receptor (hDOR) is known to play an important role in the binding of delta-selective ligands. In particular, mutation of three amino acids (Trp(284), Val(296), and Val(297)) to alanine significantly diminished delta-opioid receptor affinity for delta-selective ligands. To assess the changes in conformation accompanying binding of the endoge...

Reseña de: MARTÍ MARCO, M. R. (ed.), Friedrich Schleiermacher: teoría hermenéutica completa. Madrid: Instituto Juan Andrés de Comparatística y Globalización, 2019, pp. 281.

The variability of gene expression signatures across tumor sites has been previously documented [Stewart et al. 2017]. However, how this information can be exploited in practice is less obvious. To better understand the links between gene-based risk scores and sampling, we performed morphology-based RNA extraction studied scores' their relative prognostic value comparison with whole-tumor sampl...

Appropriate precautions in the case of flood occurrence often require long lead times (several days) in hydrological forecasting. This in turn implies large uncertainties that are mainly inherited from the meteorological precipitation forecast. Here we present a case study of the extreme flood event of August 2005 in the Swiss part of the Rhine catchment (total area 34 550 km2). This event caus...

Taiki Ogasa, Yuki Nakagawa, Shuji Isotani, Kenta Futamura, Manabu Okada, Takahisa Hiramitsu, Norihiko Goto, Shunji Narumi, Yoshihiko Watarai. Korean J Transplant 2022;36:281. https://doi.org/10.4285/ATW2022.F-4242

The cell surface proteoglycan on normal murine mammary gland (NMuMG) epithelial cells consists of a lipophilic domain, presumably intercalated into the plasma membrane, and an ectodomain that binds via its glycosaminoglycan chains to matrix components, is released intact by proteases and is detected by monoclonal antibody 281-2. The antibody 281-2 also detects a proteoglycan in the culture medi...