Sand culture using radish (Raphanus sativus L.) and hydroponics using carrot (Daucus carota L.) were conducted to examine root-uptake of carbon and its assimilation in the form of 14C-acetic acid. 14C-acetic acid (1, 2-14C, radioactivity: 74 kBq) was added to each pot. Radishes were grown under the dark conditions or the light conditions for 24 h. Carrot were grown under the light conditions af...

1. The effect of unsaturated long-chain non-esterified fatty acids (NEFA) on the human leucocyte sodium-pump was studied in vivo. 2. Plasma NEFA level was raised acutely from 0.28 (SD 0.10) to 2.54 (SD 0.48) mmol/l by infusion of 'Intralipid 20%' (trademark) at 90 ml/h with heparin, and the human leucocyte 22Na efflux rate constants were studied in eight normal weight males. 3. After 3 h, there...

1. Protoveratrine A increased the release of y-aminoPHlbutyrate from small slices of rat cerebral cortex. This effect increased with increasing protoveratrine concentration, reaching a maximum at 100,M. 2. Removal of Ca2+ from the superfusing medium did not change the increase in release due to 10,uM-protoveratrine; however, the Ca2+ antagonists, compound D-600, La3+, Mn2+, Mg2+ and also high C...

Abbreviations: ECF and ICF, extracellular and intracellular fluid respectively. drinking water. Sodium distribution in isolated mesenteric arterial wall was studied with the aid of 22Na, which was continuously washed out. Data were analysed by digital computer simulation without recourse to ancillary chemical measurements of Introduction extracellular space. 2. A three-compartment model consist...

The development of the Na/H antiporter was studied in renal brush border membrane vesicles (BBMV) from fetal and adult rabbits using isotopic and fluorescent techniques. The kinetics of the antiporter studied by 22Na+ uptake revealed that the Vmax was only 25% of that in the adult; however, the Km's for Na+ were not significantly different. These data were confirmed by a fluorescent assay using...

SUMMARY Cultured human neuroblastoma cell lines were assayed for biochemical characteristics of neuronal function. The time course of 22Na@ uptake and inhibition of uptake by tetrodotoxin sup ported this. SK-N-MC had no veratridine-dependent 22Na@ uptake. Tyrosine hydroxylase (EC 1.14.10.), glutamic acid decarboxylase (EC 4.1 .1.15), and acetyicholine contents in neuroblastoma cells were compar...

Changes in the movements of Na+, K+, and Ca+2 across rabbit neutrophils under conditions of lysosomal enzyme release have been studied. We have found that in the presence of cytochalasin B, the chemotactic factor formyl methionyl leucyl phenylalanine (FMLP) induces within 30 s large enhancements in the influxes of both 22Na+ and 45Ca+2 and an increase in the cellular pool of exchangeable calciu...

We studied the pathways for HCO-3 transport in basolateral membrane vesicles (BLMV) purified from rat medullary thick ascending limbs (MTAL). An inward HCO-3 gradient in the presence of an inside-positive potential stimulated the rate of 22Na uptake minimally and did not induce a 22Na overshoot, arguing against the presence of electrogenic Na+-HCO-3 cotransport in these membranes. An inside-aci...

The IRIS group at IFIC-Valencia has developed a second version of Compton camera prototype for hadron therapy treatment monitoring, with the aim improving performance respect to its predecessor. system is composed three Lanthanum (III) bromide (LaBr3) crystals coupled silicon photomultipliers (SiPMs). detector energy resolution been improved 5.6% FWHM 511 keV and an angular 8.0° obtained. Image...

The Na+/H+ antiport is an important regulator of cellular volume, pH and Na+ concentration in mammalian cells. The stoichiometry of this antiporter has previously been shown to be a 1:1 exchange of internal H+ for external Na+. We have investigated this stoichiometry in human leucocytes by using a novel intracellular pH-clamping technique and measuring 22Na+ influx and H+ efflux in the same cel...