CONTEXT Hepatocellular carcinoma (HCC) is one of the most common cancers in the world but with a striking geographical variation in incidence; most of the burden is in developing countries. This geographic variation in HCC incidence might be due to geographic differences in the prevalence of various etiological factors. EVIDENCE ACQUISITION Here, we review the epidemiological evidence linking...

A study of the effect in rats of dichlorodiphenyl trichloroethane (DDT) on hepatocarcinogenesis that is initated by aflatoxin B1 (AFB1). In the first experiment, Buffalo rats were given a single oral dose of AFB1 (5 mg/kg) followed by dietary DDT (100 ppm) for 20 weeks. Neoplastic nodules were observed in 1 of the 14 AFB1-exposed rats, compared with 3 of the 19 rats in the AFB1/DDT group. In th...

The monkey CYP1A1 has been expressed in BALB 3T3 A31-1-1 cells and the expressed proteins were assayed for their capacity to activate aflatoxin B1 (AFB1) and benzo[a]pyrene (B[a]P). The transformed cells were approximately 5.4- and 4.7-fold more sensitive to AFB1 and B[a]P than the parental cells, respectively. These results indicate that monkey CYP1A1 cDNA encoded a functional protein and that...

mycotoxins are secondary metabolites produced by fungi following their infestation of food crops.one of the effective approaches to control aflatoxicosis is the use of adsorbents. the aim thusfollowed in the present study was a comparison of various adsorbents viz. bentonite, zeolite, vs. anorganic commercial adsorbent (mycosorb) and a mineral – organic commercial adsorbent (biotox)regarding th...

Aflatoxin B1 (AFB1) is a secondary metabolite produced by Aspergillus flavus and A. parasiticus, known carcinogen in humans animals. High voltage atmospheric cold plasma (HVACP) technology has already shown promise to decontaminate AFB1 food feed. This study aimed investigate the cytotoxicity of after HVACP treatment. (100 ?M) was treated at 85 kV with for 0, 2, 5, 10, 20 min. HepG2 cells were ...

The covalent binding of photoactivated aflatoxin B1 ((AFB1) to DNA and its effect on template activity have been investigated. AFB1 in its ground state complexes more preferentially with denatured DNA than with native DNA. The covalent linkage between 3H-AFB1 and DNA under near-ultraviolet light irradiation shows 1 AFB1 per 529 Pi of calf thymus DNA. The photoaddition also induces a conformatio...

The association between murine cytochrome P3-450 and hepatic aflatoxin B1-4-hydroxylase, a cytochrome P-450-dependent enzyme which converts aflatoxin B1 (AFB1) to aflatoxin M1 (AFM1), was examined by (a) purification of the cytochrome P-450 which preferentially metabolizes AFB1 to AFM1; (b) isolation of the specific cDNA clone; and (c) correlating induction of transcriptional activation of the ...

Modulation of DNA adduct formation by pre-existing adducts was examined in synthetic oligonucleotides and genomic DNA (calf thymus); genotoxins studied were N-acetoxy-acetylaminofluorene (N-AcO-AAF), aminofluorene (AF), aflatoxin B1-8,9-epoxide (AFB1-8,9-epoxide), and dimethylsulfate (DMS). Oligodeoxynucleotides containing either guanine-C8-AAF (Gua-C8-AAF) or Gua-C8-AF adducts and a neighborin...