recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...

background: the aim of this study was to evaluate the protein spots of excre-tory - secretory products of fasciola hepatica using two dimension electrophoresis method in the presence and absence of triclabendazole drug which can be consid-ered to detect the target protein of the drug. methods: f. hepatica parasites were collected from infected cattle livers, divided in two groups and cultivated...

Background: Denileukin diftitox (trade name, Ontak) is the first recombinant immunotoxin (IM), in which the binding domain of diphtheria toxin has been replaced by the amino acid sequence of human interleukin-2 (DT389IL-2) using genetic engineering. Purity, stability, and structural property of the protein are critical factors for the scale-up production of this fusion protein. In this IM, loca...

twenty eight lactating buffaloes were used in a completely randomized design with 2×2 factorial arrangement of four experimental diets including low protein–low energy (lp-le), low protein–high energy (lp-he), high protein–low energy (hp-le) and high protein–high energy (hp-he). results showed that the hp-he diet recorded the highest digestibility coefficients of cp, ee, nfe, nutritive values, ...