Relationships among the ecdysozoans, or molting animals, have been difficult to resolve. Here, we use nearly complete 28S+18S ribosomal RNA gene sequences to estimate the relations of 35 ecdysozoan taxa, including newly obtained 28S sequences from 25 of these. The tree-building algorithms were likelihood-based Bayesian inference and minimum-evolution analysis of LogDet-transformed distances, an...

tropical theileriosis is a tick-transmitted disease which caused by obligative intracellular protozoan theileria annulata. diagnosis is usually based on identification of parasite in giemsa stained smears of peripheral blood, spleen or lymph nodes. today, the molecular methods with high sensitivity and specificity are used for diagnosis of infected and carrier animals, epidemiological and phylo...

Considering the importance of Theileria equi infection in horse breeding industry and marketing, in the present study, we aimed to determine the prevalence of T. equi among dromedaries in central Iran, where a considerable number of camels and horses are raised and equine theileriosis is quite prevalent. For this purpose, a total of 161 blood samples from camels were examined in terms of T. equ...

cryptosporidium is an obligate intracellular protozoan parasite infecting a wide range of hosts. the current study investigated the genetic profile of cryptosporidium species in calves in liverpool, england. fifty-two calve fecal samples were collected from a farm and initially screened by auramine phenol, modified ziehl-neelsen and elisa. pcr analysis of 18s rrna gene was carried out for the p...

Tea plants [Camellia sinensis (L.) O. Kuntze] are an important leaf-type crop that are widely used for the production of non-alcoholic beverages in the world. Exposure to excessive amounts of heavy metals adversely affects the quality and yield of tea leaves. To analyze the molecular responses of tea plants to heavy metals, a reliable quantification of gene expression is important and of major ...

We describe the new brackish tanaidid species Sinelobus kisui sp. nov. from Hagi, Yamaguchi, Japan. is similar to S. barretti and vanhaareni in having antennal article 2 with one outer distal seta, dorsodistal crotchet on pereopods 3 carpi shorter than half propodus length, pereopodal 2–6 five crotchets, but differs them (1) inner of two ventro-subdistal circumplumose setae maxillipedal endite ...

An initial survey of sequences of PCR-amplified portions of the 18S rRNA genes from a community DNA clone library, prepared from an algal mat in a thermal, acidic stream in Yellowstone National Park, WY, USA, revealed among other sequences, several that matched Vahlkampfia. This finding prompted further investigation using primers specific for Naegleria. Sequences from a subsequent DNA clone li...

Analysis of multiple marker genes using metabarcoding environmental DNA (eDNA) can offer information greater than that from sequencing single genes, such as responses across the phylogenetic tree to gradients (Cordier et al. 2019). Furthermore, regions same gene be sequenced improve resolution (Fuks 2018). However, separate amplification reactions and library preparation steps for each costly t...