There are a number of applications of molecular markers in agriculture such as assessing genetic diversity, generating DNA fingerprints and developing markers linked to a trait of interest, etc. Arbitrarily Primed-Polymerase Chain Reaction (AP-PCR) in which template DNA is amplified using single arbitrary primers of 10 base in length is a widely used technique. The objectives of this investigat...

An attempt has been made to analyze the distribution of the beta-lactoglobulin genotype in swamp buffaloes and Murrah buffaloes utilizing polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Blood samples were taken from 50 swamp buffaloes and 50 Murrah buffaloes. The DNA was extracted by the phenol-chloroform method. The PCR-RFLP was performed using beta-lactoglobulin ...

The present work dealt with RAPD markers for genetic diversity comparison estimation in twenty three cotton varieties of Bt and non-Bt cotton cultivars belonging to two cultivated species of cotton in Andhra Pradesh state, India.Variations among twenty three different cotton varieties were analyzed with selected 40 RAPD primers using the polymerase chain reaction (PCR). All the primers produced...

Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic diversity in this species. Fifty-six polymorphic primers (29 R...

INTRODUCTION Interleukin-18 (IL-18) is a Th1 cytokine, which is postulated to play a role in systemic lupus erythematosus (SLE). Two single nucleotide polymorphisms (SNPs) in the IL-18 promoter gene region were found to influence the quantitative expression of the IL-18 protein. The aim of this study was to determine whether IL-18 promoter gene polymorphisms are associated with SLE. MATERIALS...

OBJECTIVE To assess the association of the polymorphisms of the interleukin-18 (IL-18) gene with rheumatoid arthritis (RA) and with risk factors for cardiovascular diseases (CVD). METHODS This sample comprised 97 patients with RA and 151 healthy controls. In the patients, risk factors for CVD were analyzed, such as cholesterol levels, arterial hypertension, smoking habit, C-reactive protein (...

The RAPD method (Random Amplified Polymorphic DNA) was used for identifying and mapping new molecular markers in pea. RAPD analysis of various cultivars and lines of pea was carried out using 10-mer random primers. The presence of multiple polymorphism between cultivars and lines was revealed; at least one fragment for any given primer was present in the DNA of one form of pea and absent in the...

The classification of bamboos based on floral morphology and reproductive characters is very hard due to erratic flowering behavior and unusually long reproductive cycle. The application of reliable and effective DNA molecular markers is highly essential to address this problem. In the present investigation, inter-simple sequence repeats (ISSR) markers were employed to study phylogenetic relati...

Random amplified polymorphic DNA markers were used to evaluate the genetic diversity in a representative population of Terminalia pallida from six different geographical regions of chittoor district. 20 OPA primers were used for the RAPD analysis. A total of 33 bands yielded from 7 reproducible primers, with a mean of 4.28 amplified bands per primer. The plant species showed 69.5% polymorphism....