Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone li...

Objective(s) Rapid tests for detection of Streptococcus agalactiae or Group B Streptococci (GBS) at the onset of labor are needed to permit early intrapartum antibiotic prophylaxis. This study aimed to evaluate the PCR assays targeting the 16S ribosomal RNA gene (16S rDNA) for detection of the GBS in comparison with a specific culture method. Materials and Methods Two swabs were used to obtain ...

Seventeen isolates of Bartonella henselae from the region of Freiburg, Germany, obtained from blood cultures of domestic cats, were examined for their genetic heterogeneity. On the basis of different DNA fingerprinting methods, including pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, repetitive extragenic palindromic (REP) PCR, and arbitrari...

introduction and aims: until today human stomach is the only recognized habitat of h. pylori . however, recruitment of dna-based methods has made possible the detection of h. pylori in water and oral cavity, thus suggesting faecal-oral and oral-oral routes for transmission of h. pylori , respectively. in this study yeast has been proposed as a common vector for transmission of h. pylori . thus ...

Our purpose was to develop a rapid, inexpensive method of diagnosing the source of fecal pollution in water. In previous research, we identified Bacteroides-Prevotella ribosomal DNA (rDNA) PCR markers based on analysis. These markers length heterogeneity PCR and terminal restriction fragment length polymorphism distinguish cow from human feces. Here, we recovered 16S rDNA clones from natural wa...

objective(s) rapid tests for detection of streptococcus agalactiae or group b streptococci (gbs) at the onset of labor are needed to permit early intrapartum antibiotic prophylaxis. this study aimed to evaluate the pcr assays targeting the 16s ribosomal rna gene (16s rdna) for detection of the gbs in comparison with a specific culture method. materials and methods two swabs were used to obtain ...