We have used isolated perfused rat livers to examine the intracellular processing of 125I-epidermal growth factor (EGF) and to determine where in the endocytic pathway the hydrolases which degrade EGF are acting. Following uptake of 125I-EGF at 37 or 16 degrees C, subcellular fractions enriched in endosomes and lysosomes were isolated, and their 125I-EGF content was examined by reverse-phase hi...

Slow motion of a thin plate at a finite angle of attack in a rotating container filled with a viscous incompressible fluid is analysed. The Ross by and Ekman numbers are assumed to be small. The solution method is developed by studying horizontal translation of an elliptical plate. The plate is shown to carry a stagnant Taylor column with it as it moves. Detailed analysis of the structure of th...

The cellular fate of insulin receptors in isolated rat adipocytes was studied by using a biologically active photosensitive insulin derivative, B2(2-nitro-4-azidophenylacetyl)-des-PheB1-insulin (NAPA-DP-insulin), to photoaffinity label the insulin receptors. Insulin receptors specifically labeled with 125I-labeled NAPA-DP-insulin were identified by NaDodSO4/polyacrylamide gel electrophoresis an...

Measurements were made of the femoral shaft/neck angle (CCD angle) in 106 human foetuses, aged from 16 to 38 hbd, using a "FEM-GEO_03" computer program. The values of the CCD angle in the group under examination were: mean = 140.48 degrees, SD = 6.95 degrees, max = 157.90 degrees, min = 113.93 degrees. No significant differences were found in CCD angle size between male and female foetuses or b...

Decimal reduction time (time to inactivate 90% of the population) (D) values of Bacillus anthracis spores in milk ranged from 3.4 to 16.7 h at 72 degrees C and from 1.6 to 3.3 s at 112 degrees C. The calculated increase of temperature needed to reduce the D value by 90% varied from 8.7 to 11.0 degrees C, and the Arrhenius activation energies ranged from 227.4 to 291.3 kJ/mol. Six-log-unit viabi...

Despite its vector importance little attention is given to Aedes aegypti embryonic development. In this study, temperature influence on time course of Ae. aegypti larvae hatching and egg viability were evaluated. The dormancy state at the end of embryogenesis could be interrupted with a proper stimulus. Temperatures tested ranged between 12-36 degrees C; the maximum temperature limit is 35 degr...

We analysed the long-term clinical and radiological results of 63 uncemented Low Contact Stress total knee replacements in 47 patients with rheumatoid arthritis. At a mean follow-up of 12.9 years (10 to 16), 36 patients (49 knees) were still alive; three patients (five knees) were lost to follow-up. Revision was necessary in three knees (4.8%) and the rate of infection was 3.2%. The mean clinic...

The primary objectives of this study were to determine (1) whether rapid cold-hardening (RCH) preserves reproductive behaviors during modest cooling, (2) whether increased mating success at a lower temperature comes at the cost of decreased performance at a higher temperature and (3) whether RCH is associated with an elevated metabolic rate. Drosophila melanogaster (Diptera: Drosphilidae) were ...

Animals select their optimal environmental temperature, even when faced with alternatives that differ only slightly. This behavior is critical as small differences in temperature of only several degrees can have a profound effect on the survival and rate of development of poikilothermic animals, such as the fruit fly. Here, we demonstrate that Drosophila larvae choose their preferred temperatur...

The growth kinetics of two psychrotolerant Antarctic bacteria, Hydrogenophaga pseudoflava CR3/2/10 (2/10) and Brevibacterium sp. strain CR3/1/15 (1/15), were examined over a range of temperatures in both batch culture and glycerol-limited chemostat cultures. The maximum specific growth rate (mu max) and Ks values for both bacteria were functions of temperature, although the cell yields were rel...