Bacterial DNA-dependent RNA polymerase (RNAP) has subunit composition beta'betaalpha(I)alpha(II)omega. The role of omega has been unclear. We show that omega is homologous in sequence and structure to RPB6, an essential subunit shared in eukaryotic RNAP I, II, and III. In Escherichia coli, overproduction of omega suppresses the assembly defect caused by substitution of residue 1362 of the large...

Perioperative blood loss was compared in 136 patients (2 groups of 68 patients) who underwent total knee arthroplasty (TKA). Blood loss was significantly lower when using a computer-navigated technique in comparison to a method employing intramedullary femoral rods. Total blood loss was calculated from body weight, height and haematocrit change, using a model that has been shown to reliably est...

This prospective study was conducted in 6 hospitals in Edmonton, Canada to determine the factors associated with obtaining sputum for culture and the effect of sputum culture on the management of patients with community-acquired pneumonia (CAP). Participants were 1362 adults who were hospitalized with CAP. Sputum was obtained from 539 (39.6%) patients, of which 507 (94.1%) were good quality, ac...

A chitosanase produced constitutively by Bacillus sp. MET 1299 was purified by SP-Sephadex column chromatography. The molecular weight was estimated to be 52 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Optimal enzyme activity was observed at a pH of 5.5 and temperature of 60 degrees C. The purified chitosanase showed high activity on 90% deacetylated colloidal c...

ObjectivesTo determine the prevalence and associated factors of diabetes mellitus (DM) among tuberculosis (TB) cases in Brunei Darussalam.MethodsWe conducted a retrospective cohort study all TB patients registered between 2013 2018. Collected data include sociodemographics, comorbidities, information related to their diagnosis treatment outcomes. Associated having DM were investigated using log...

Abstract Copper vanadate (CV) and manganese (MV) exhibit magnetic optical properties that have drawn the attention. Due to CV polymorphism phase multiplicity, is common exist as mixed phases. In this study, nanostructures of mixed-phase CVs with MV were synthesized hydrothermally followed by calcination at 400 °C, Mn mole fractions 0.0, 0.4, 0.6, 1.0. The uncalcined calcined Mn-Cu vanadates (MC...

Bloom date (BD) in peach is determined by the dynamic relationship between chilling (CR) and heat requirement (HR) fulfilment during dormancy. Understanding these thermal requirements would enable breeders to adapt new cultivars variable climates. Among three traits, HR least investigated, with genetic variability germplasm interaction HR, CR BD still mostly unknown. Therefore, we investigated ...