While only four globally important rotavirus G serotypes (1 to 4) have been documented, many studies suggest that serotype G9 viruses may be widely distributed and more important than previously recognized. We have evaluated 10 serotype G9 rotavirus-neutralizing monoclonal antibodies (MAbs) directed to VP7, which bound by direct enzyme immunoassay (EIA) to P1A[8], G9 rotaviruses F45, WI61, and ...

The beetle Cucujus clavipes is found in North America over a broad latitudinal range from North Carolina (latitude approximately 35 degrees N) to near tree line in the Brooks Range in Alaska (latitude, approximately 67 degrees 30' N). The cold adaptations of populations from northern Indiana (approximately 41 degrees 45' N) and Alaska were compared and, as expected, the supercooling points (the...

this study aimed to evaluate freeze-dried sera as an alternative to non-freeze dried for detection of anti-leishmania infantum antibodies over the course of 11 months using the direct agglutination test (dat).altogether, 60 serum samples (30 from humans and 30 from dogs) were collected from various geographical locations in iran. all the collected sera were pooled and each pooled serum sample c...

The freeze-drying encapsulation process was used to encapsulate dragon fruit peel extract with three distinct wall materials: maltodextrin, gum arabica, and gelatin. modeled using a feed forward back propagation artificial neural network four, eleven, four neurons in the input, hidden, output layers. Three of input were concentrations material (g), while fourth ultrasonication power. efficiency...

this study was undertaken to determine the concentrations of metals in the muscle and exoskeletonof the crayfish orconectes limosus in order to compare them with other species of crayfish and crustaceans.in addition, we analyzed the correlation between the concentrations of the same element in the exoskeleton andthe meat and different elements accumulated in the same tissue. crayfish individual...

Today, with the understanding of favourable effects fruit consumption on health, pomegranate has increased gradually, and solution methods were sought to evaluate peel seed parts that emerge after is sorted. In this study, different drying processes (microwave, oven, freeze-drying) extraction solvents (ethanol methanol) antioxidant antidiabetic activities press wastes investigated. While highes...

Daylily (Hemerocallis fulva Linn.) flowers were hot air-dried and freeze-dried after harvest. Antioxidant properties of water and ethanol extracts prepared from these dried flowers were evaluated in terms of total antioxidant activity, reducing capacity, and metal chelating activity. Extracts from daylily flowers exhibited strong antioxidant activity. Ethanol was more efficiency to extract anti...

OBJECTIVE Malformations of cortical development are frequent causes of human refractory epilepsy. The freeze-lesion model in rats shows histopathological features similar to those found in human polymicrogyria. Previous studies reported in vitro hyperexcitability in this model, but in vivo epileptogenicity has not been confirmed. METHODS Neocortical freeze lesions were induced in Sprague-Dawl...

Tomato ketchup, barbeque sauce, sweet chili sauce, and spaghetti sauce were gamma irradiated at 0, 1, 5, and 10 kGy. Electron spin resonance (ESR) technique was used to characterize the irradiated sauces, targeting radiation-induced cellulose radicals and using a modified sample pretreatment method. The samples were first washed with water, and then the residues were extracted with alcohol. The...