*A.C. Fernandes and V. Uytterhoeven contributed equally to this paper. Correspondence to Patrik Verstreken: [email protected] Abbreviations used in this paper: ANOVA, analysis of variance; CSP, cysteine string protein; Dor, Deep orange; EJC, excitatory junctional current; EMS, ethyl methanesulfonate; ERG, electroretinogram; FT, fluorescent timer; HOPS, homotypic fusion and vacuo...

The ab initio determination of the leading long-range term of pairwise additive dispersive interactions, based on the independent analysis of the response properties of the interacting objects, is here considered in the case where these are part of a periodic system. The interaction of a nitrogen molecule with a thin film of hexagonal BN has been chosen as a case study for identifying some of t...

The transmembrane protein Crumbs (Crb) functions in apical polarity and epithelial integrity. To better understand its role in epithelial morphogenesis, we examined Crb localization and dynamics in the late follicular epithelium of Drosophila. Crb was unexpectedly dynamic during middle-to-late stages of egg chamber development, being lost from the marginal zone (MZ) in stage 9 before abruptly r...

drugs that could be used in conjunction with surgery to treat this debilitating neurological disease. receptor may be new therapeutic targets for developing 1 the fetal mice. These results suggest that LPA and its LPA receptor antagonist blocked the ability of LPA to induce hydrocephalus in 1 came with their demonstration that an LPA receptor, they were unable to induce hydrocephalus. The key f...

Correspondence to Andrew M. Fry: [email protected] A. Knebel’s present address is Medical Research Council Protein Phosphorylation Unit, The Sir James Black Center, Dundee DD1 5EH, Scotland, UK. Abbreviations used in this paper: HSP, heat shock protein; IF, immunofluorescence; IP, immunoprecipitate; KESTREL, kinase substrate–tracking and elucidation; LSB, low salt buffer; MT, microtubule; NEBD, nuc...

The regulation of columella stem cell activity in the Arabidopsis root cap by a nearby organizing centre, the quiescent centre, has been a key example of the stem cell niche paradigm in plants. Here, we investigate interactions between transcription factors that have been shown to regulate columella stem cells using a simple quantification method for stem cell activity in the root cap. Genetic ...

1 The Rockefeller University Press $30.00 J. Cell Biol. https://doi.org/10.1083/jcb.201610056 How do we define organoids and 3D cultures? That functional differentiation is dependent on 3D architecture has become accepted recently. Many papers over the last 50 years have shown that cells cultured in 2D are not representative of the in vivo situation. Structurally, 2D cultures do not provide the...

Myogenesis involves the stable commitment of progenitor cells followed by the execution of myogenic differentiation, processes that are coordinated by myogenic regulatory factors, microRNAs and BAF chromatin remodeling complexes. BAF60a, BAF60b and BAF60c are structural subunits of the BAF complex that bind to the core ATPase Brg1 to provide functional specificity. BAF60c is essential for myoge...