The present study was conducted at the Biotechnology Laboratory, Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur during the period from September 2004 to June 2005 to investigate the effect of different concentrations of BAP and NAA on virus free plant regeneration, shoot multiplication and different concentrations of IBA and IAA on in vitro root formation of ...

Eclipta alba is a medicinal herb, also known as Bhringraj belonging to family Asteraceae. The plant has hepatoprotective, antimicrobial and hair growth promoting properties. The effect of various phytohormones on in-vitro callus induction and shoot regeneration in Eclipta alba from leaf explant was evaluated. The sterilized explants were inoculated on MS medium supplemented with different conce...

Shoot multiplication of Bupleurum distichophyllum was achieved from the nodal and shoot tip explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response was from axillary shoots and the highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of axillary shoot proliferation was fou...

fig trees are threatened by the attack of fig mosaic disease (fmd) on leaves and fruits caused by viruses of several genera. shoot-tip culture is a convenient method for viral sanitation. for this purpose, a reliable protocol for rapid in vitro propagation was developed with shoot-tips of three major tunisian local fig (ficus carica l.) varieties zidi (zdi), soltani (sni), bither abiadh (ba) an...

in vitro propagation of az x m9 new apple rootstock resulted of breeding program of vegetative apple rootstock in iran was investigated. nodal explants were inoculated in murashige and skoog (ms) medium supplemented with various concentrations of 6-benzylaminon purine (bap) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (naa) (0.1 mg/l). we examined the effect of different concen...

Plants growth regulators, especially auxins and cytokinins are critical for in-vitro plant regeneration. The effect of plant growth regulators on the in-vitro propagation of Senna alata (Linn) was investigated. Mature seeds were collected from the medicinal garden of National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan (Lat.7022I N and long.3050I E). Seeds of the species we...

A reliable regeneration system for faba bean has been difficult to establish and therefore, the genetic improvement of Vicia faba L. was delayed. The paper describes a method of somatic embryo induction in callus of V. faba. Two Egyptian faba bean cultivars 'Giza 2' and '24 Hyto' were used. Callus was induced from epicotyls and shoot tips cultured on MS or Gamborg medium supplemented with 3% su...