A relatively rapid procedure is described for the spectrophotometric determination of total tocopherol in red blood cells (RBC) based on a modification of the original Emmerie-Engel reaction. The critical feature in this method is the presence of a large amount of an added antioxidant, pyrogallol or ascorbic acid, during the saponification and extraction stages and the use of thin-layer chromat...

OBJECTIVE To assess vitamin E levels in the breast milk, analyzing the prematurity and the birth weight influence in α-tocopherol concentration of colostrum milk. METHODS Cross-sectional study, in which the colostrum was collected from 93 nursing mothers in a public maternity of Natal, Rio Grande do Norte, Northeast Brazil. The newborns were classified based on gestational age and birth weigh...

The aims of this research was to investigate effect alpha-tocopherol on spermatozoa death in form apoptosis and necrosis rats (Rattus norvegicus) exposed 2,3,7,8- tetrachlorodibenzo-p-dioxin. Male were administered TCDD experimental groups. Five groups a combination alpha- tocopherol designed as follows; 0.5 ml corn oil (control negative group, K-), 700 ng/kg/d (treatment control), 77 (Group P1...

A rapid, selective and sensitive ultra-performance liquid chromatography method has been developed for the detection and quantification of tocopherols and retinol in human plasma. Alpha-tocopherol, gamma-tocopherol and retinol are assayed using fluorescence detection. Excitation/emission wavelengths are 295/330 nm and 325/470 nm for the analysis of both tocopherols and retinol, respectively. Re...

In this study, moisture content, free fatty acids, peroxide, iodine value, unsaponifiable matter, saponification value, fatty acid composition and tocopherol contents of wheat germ oil obtained by SC-CO2 extraction and cold press technology were investigated. Moisture, free fatty acid (FFA), peroxide value, iodine value, unsaponifiable matter and saponification value of cold pres...

Background Radiotherapy and chemotherapy could affect spermatogenesis process, so it is important to preserve fertility of patients who suffer from cancer disease. Cryopreservation of SSCs is considered as a helpful procedure for preserving male fertility in cancer patients. It seems that antioxidants can decrease ROS formation and reduce side-effects of cryopreservation on cells. In this study...

Twelve pumpkin cultivars (Cucurbita maxima D.), cultivated in Iowa, were studied for their seed oil content, fatty acid composition, and tocopherol content. Oil content ranged from 10.9 to 30.9%. Total unsaturated fatty acid content ranged from 73.1 to 80.5%. The predominant fatty acids present were linoleic, oleic, palmitic, and stearic. Significant differences were observed among the cultivar...

Wheat germ represents approximately 3% of the grain and it contains 8-14% oil, which is a rich source of tocopherols (vitamin E) and polyunsaturated fatty acids, mainly linoleic acid. The present work shows the influence of temperature (27oC and 45oC) and storage time (maximum 35 days) of the wheat germ on the concentration of tocopherol in the oil. Their effect on other quality parameters was ...

The aim of this study was to investigate the effect of different types of tomato processing on contents of lycopene, beta-carotene, and alpha-tocopherol. Samples of tomato sauce, tomato soup, baked tomato slices, and tomato juice were taken at different times of heating, respectively, after each step of production. HPLC was used to analyze contents of carotenoids and vitamin E. Due to the loss ...

Much data has accumulated supporting a proatherogenic role for oxidized low density lipoprotein (Ox-LDL). Micronutrient antioxidants such as alpha-tocopherol, the principal lipid-soluble antioxidant, assume potential significance because levels can be manipulated by dietary measures without resulting in side effects. Co-incubation of LDL in vitro with alpha-tocopherol inhibits its oxidative mod...