Sir, Enterobacteriaceae producing metallo-b-lactamases (MBLs), and particularly VIM-type MBLs, have frequently been implicated in hospital outbreaks across Europe, blaVIM genes having been linked to Tn402 derivatives, epidemic plasmids (IncN, IncI1, IncHI2) and occasionally with particular Enterobacteriaceae clones. VIM enzymes have been classified in three clusters (VIM-1, VIM-2 and VIM-7) acc...

Introduction: Non-fermentative Gram-negative bacilli (NFGNB) are an important cause of healthcare-associated infections, and theyare primarily opportunistic pathogens.Aim objectives: To isolate characterize the non-fermenting from various clinical samples ina tertiary care center. detect virulence markers drug resistance using molecular methods.Material methods: were identified standardbacterio...

Cephalosporin-resistant Escherichia coli has been increasingly reported worldwide. In this study, 32 cephalosporin resistant E. coli isolates identified from cancer patients in Cairo, Egypt in 2009-2010 were analyzed. Twenty-three were of phylogenetic group D, seven A and one each B1 and B2. By rep-PCR 15 phylogroup D isolates were grouped in four clusters, one with sequence type (ST) 405 and t...

Clinical strains of Enterobacter were isolated from Cumana's Central Hospital in Venezuela, and classified as E. cloacae (21), E. aerogenes (7), E. intermedium (1), E. sakazakii (1) and three unclassified. The strains showed high levels of resistance, especially to SXT (58.1%), CRO (48.8%), CAZ (46.6%), PIP (46.4%), CIP (45.2%) and ATM (43.3%). This is the first report for South America of blaV...

Methods This study describes the molecular characterization of 15 Pseudomonas aeruginosa strains, isolated from endoscopic pus specimens, obtained from sinusitis cases. Susceptibility testing was performed by disk diffusion assay. The MIC of ceftazidime was determined by E test. Ceftazidime (CDM) EDTA combined disc test was used to screen for class B b lactamase production. Plasmid DNA extracti...

Resistome analysis of clinical VIM-1-producing Enterobacter cloacae strain CY01 from China revealed the presence of multiple resistance determinants. Two resistance plasmids were identified in CY01. The pCY-VIM plasmid was 14 kb in size and possessed a replicase gene (repA), a gene cluster encoding the partitioning function (parABC), and a carbapenemase gene (blaVIM-1). Another 5.9-kb plasmid, ...