Wehr M, Hostick U, Kyweriga M, Tan A, Weible AP, Wu H, Wu W, Callaway EM, Kentros C. Transgenic silencing of neurons in the mammalian brain by expression of the allatostatin receptor. J Neurophysiol 102: 2554–2562, 2009. First published August 19, 2009; doi:10.1152/jn.00480.2009. The mammalian brain is an enormously complex set of circuits composed of interconnected neuronal cell types. The ana...

The mammalian brain is an enormously complex set of circuits composed of interconnected neuronal cell types. The analysis of central neural circuits will be greatly served by the ability to turn off specific neuronal cell types while recording from others in intact brains. Because drug delivery cannot be restricted to specific cell types, this can only be achieved by putting "silencer" transgen...

Recently, we have described a regulatory system that allows the stringent control of individual gene activities in higher eukaryotic cell lines (1), in plants (2) and in animals (3,4). The essential components of this system are (i) an RNA polymerase II minimal promoter placed downstream of multiple operator sequences (tetO) of the Escherichia coli TnlO tetracycline resistance operon and (ii) a...

This short technical report describes the generation and characterization of a bioluminescence reporter mouse that is engineered to detect and longitudinally monitor the expression of doxycycline-induced constitutively active β-catenin. The new responder transgenic mouse contains the TetO-ΔN89β-CatTMILA transgene, which consists of the tet-operator followed by a bicistronic sequence encoding a ...

The large variability in mRNA and protein levels found from both static and dynamic measurements in single cells has been largely attributed to random periods of transcription, often occurring in bursts. The cell cycle has a pronounced global role in affecting transcriptional and translational output, but how this influences transcriptional statistics from noisy promoters is unknown and general...

O propósito deste artigo é analisar, sob diferentes cenários, a trajetória da dívida pública com uma regra do teto dos gastos correntes governo. Utilizou-se um modelo Dinâmico Estocástico de Equilíbrio Geral considerando dois cenários distintos, assumindo o cumprimento ou não nova fiscal por parte No contexto em que governo cumpriu teto, resultado primário caiu forma significativa e cresceu per...

Conditional expression of short hairpin RNAs (shRNAs) to knock down target genes is a powerful tool to study gene function. The most common inducible expression systems are based on tetracycline-regulated RNA polymerase III promoters. During the last years, several tetracycline-inducible U6 and H1 promoter variants have been reported in different experimental settings showing variable efficienc...