نتایج جستجو برای: سیاستهای hrm

تعداد نتایج: 4806  

Journal: :Genetics and molecular research : GMR 2016
J Q Deng B Q Liu Y Wang W Liu J F Cai R Long W H Li

Currently, the widely used automated capillary electrophoresis-based short tandem repeat (STR) genotyping method for genetic screening in forensic practice is laborious, time-consuming, expensive, and technically challenging in some cases. Thus, new molecular-based strategies for conclusively identifying forensically relevant biological evidence are required. Here, we used high-resolution melti...

2017
Lucas B. Harrison Nancy D. Hanson

Escherichia coli isolates belonging to the sequence type 131 (ST131) clonal complex have been associated with the global distribution of fluoroquinolone and β-lactam resistance. Whole-genome sequencing and multilocus sequence typing identify sequence type but are expensive when evaluating large numbers of samples. This study was designed to develop a cost-effective screening tool using high-res...

Journal: :Molecular and cellular probes 2014
Beata Komorowska Nicola Fiore Alan Zamorano Ruhui Li

In this study, the real-time PCR assays were combined with high resolution melting (HRM) analysis for the simultaneous detection of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV) infection in sweet cherry trees. Detection of CNRMV and CGRMV was performed in a real-time PCR using a primer set for both of them or duplex real-time PCR that included one specif...

Journal: :Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2008
Lasse S Kristensen Alexander Dobrovic

High-resolution melting (HRM) shows great promise for high-throughput, rapid genotyping of individual polymorphic loci. We have developed HRM assays for genotyping single nucleotide polymorphisms (SNP) in several key genes that are involved in methyl metabolism and may directly or indirectly affect the methylation status of the DNA. The SNPs are in the 5,10-methylenetetrahydrofolate reductase (...

ژورنال: تحقیقات دامپزشکی 2018

زمینه مطالعه: نقش مهم مولکول‏های مجتمع عمده پذیرش بافتی (MHC) اتصال به پپتید‌های پادگنی و عرضه آن‌ها به لنفوسیت‏های T است. از اینرو تنوع MHC با حساسیت و یا مقاومت در برابر بسیاری از بیماری‏های عفونی و بیماری های خودایمن مرتبط است. یک روش نوین بررسی تنوع ژنتیکی، تحلیل دقیق دمای شکافت (HRM-High Resolution Melting) DNA است که می توان آنرا برای بررسی تنوع MHC به کار برد. هدف: تمییز آلل‏ های MHC سگ ...

Journal: :international journal of hematology-oncology and stem cell research 0
farhad zaker cellular and molecular research center, iran university of medical sciences dept. of haematology, school of allied medicine, iran university of medical sciences nahid nasiri dept. of haematology, school of allied medicine, iran university of medical sciences naser amirizadeh blood transfusion research center, high institute for education and research in transfusion medicine seyed mohsen razavi hematology and oncology department, firoozgar hospital, iran university of medical sciences marjan yaghmaie hematology, oncology and stem cell transplantation research center, tehran university of medical science ladan teimoori-toolabi molecular medicine department, biotechnology research center, pasteur institute of iran

background: myelodysplastic syndromes (mdss) include a diverse group of clonal bone marrow disorders characterized by ineffective hematopoiesis and pancytopenia.it was found that down regulation of apaf1, a putative tumor suppressor gene (tsg),leads to resistance to chemotherapy and disease development in some cancers. in this study, we investigated the relation of apaf1 methylation status with...

Journal: :Applied and environmental microbiology 2007
Erin P Price Helen Smith Flavia Huygens Philip M Giffard

A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful additio...

2013
Seyed Abas HASSANI Hossein MOBARAKI Maboubeh BAYAT Shiva MAFIMORADI

BACKGROUND In this paper the real role and place of human resource (HR) in health system reform will be discussed and determined within the whole system through the comprehensive Human Resource Management (HRM) model. METHOD Delphi survey and a questionnaire were used to 1) collect HR manager ideas and comments and 2) identify the main challenges of HRM. Then the results were discussed in an ...

Journal: :The European respiratory journal 2011
K B Sriram M E Tan S M Savarimuthu C M Wright V Relan R E Stockwell B E Clarke E E Duhig I A Yang R V Bowman K M Fong

The clinical applicability of screening surgically resected nonsmall cell lung cancer (NSCLC) tumour tissue and serum for activating epidermal growth factor receptor (EGFR) mutation is unknown. Furthermore, the comparative accuracy of inexpensive EGFR mutation tests, mutant-enriched (ME)-PCR and high-resolution melt (HRM) has not been determined. Lung tumour DNA from 522 surgically resected sta...

2012
Ta-Hsien Lee Tzong-Shoon Wu Ching-Ping Tseng Jiantai Timothy Qiu

BACKGROUND Genotyping of human papillomarvirus (HPV) is crucial for patient management in a clinical setting. This study accesses the combined use of broad-range real-time PCR and high-resolution melting (HRM) analysis for rapid identification of HPV genotypes. METHODS Genomic DNA sequences of 8 high-risk genotypes (HPV16/18/39/45/52/56/58/68) were subject to bioinformatic analysis to select ...

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