A major goal in regenerative medicine is to understand and ultimately facilitate our body’s ability to repair itself following injury. As a first step toward this goal, we have begun to investigate the molecular and cellular basis of embryonic wound healing, given that embryos have the capacity to heal wounds quickly and completely. Tissue repair resembles embryo morphogenesis in several ways, ...

C calcium serves as a ubiquitous signal for acute cellular activation and for regulation of important cellular processes such as cell growth, division, differentiation, and even cell death and apoptosis. Increases in cytoplasmic calcium or calcium signals can be generated either by release of calcium from intracellular stores or by influx of calcium across the plasma membrane, but commonly by b...

Oestradiol (E(2)) accelerates oviductal transport of oocytes in cycling rats through a nongenomic pathway that involves the cAMP-PKA signalling cascade. Here we examined the role of the inositol triphosphate (IP3) and mitogen-activated protein kinase (MAPK) signalling cascades in this nongenomic pathway. Oestrous rats were injected with E(2) s.c. and intrabursally (i.b) with the selective inhib...

A major goal in regenerative medicine is to understand and ultimately facilitate our body’s ability to repair itself following injury. As a first step toward this goal, we have begun to investigate the molecular and cellular basis of embryonic wound healing, given that embryos have the capacity to heal wounds quickly and completely. Tissue repair resembles embryo morphogenesis in several ways, ...

A major goal in regenerative medicine is to understand and ultimately facilitate our body’s ability to repair itself following injury. As a first step toward this goal, we have begun to investigate the molecular and cellular basis of embryonic wound healing, given that embryos have the capacity to heal wounds quickly and completely. Tissue repair resembles embryo morphogenesis in several ways, ...

There is increasing evidence for rapid steroid action on electrolyte transport in human mononuclear leukocytes (HML). In HML, aldosterone stimulates the Na+/H+ antiporter within a few minutes. Because a variety of hormones and growth factors activate the Na+/H+ antiporter via protein kinase C and inositol phospholipids, a possible involvement of inositol-1,4,5-trisphosphate (IP3) in the rapid e...

To evaluate the relationship of inositol 1,4,5-trisphosphate (IP3) receptor-mediated signal transduction and cellular energy dynamics, we have characterized effects of nucleotides on IP3 receptor (IP3R)-mediated calcium (Ca2+) flux in purified IP3 receptors reconstituted in lipid vesicles (IP3RV) and examined hypoxia-induced augmentation of intracellular Ca2+ in intact cells. Reduced nicotinami...

The activation of a variety of cell surface receptors results in a biphasic increase in the cytoplasmic Ca2+ concentration due to the release or mobilization of Ca2+ from intracellular stores and to the entry of Ca2+ from the extracellular space. It is well established that phosphatidylinositol 4,5-bisphosphate hydrolysis is responsible for the changes in Ca2+ homeostasis. Stimulation of Ca2(+)...

UNLABELLED We investigated the effect of ketamine on inositol 1,4,5-trisphosphate (IP3) formation in rat cardiomyocytes. After the addition of 1 micromol/L ketamine, IP3 production in the presence of 0.5, 1, 5, 10, and 30 mmol/L Ca2+ significantly decreased from 537.1+/-8.3, 590.7+/-12.9, 690.6+/-7.9, 754.8+/-12.5, and 823.7+/-15.2 pmol/mg protein to 467.0+/-8.3, 483.8+/-11.0, 512.6+/-21.3, 612...

Murthy, Karnam S. cAMP inhibits IP3-dependent Ca21 release by preferential activation of cGMP-primed PKG. Am J Physiol Gastrointest Liver Physiol 281: G1238–G1245, 2001.— The singular effects and interplay of cAMPand cGMP-dependent protein kinase (PKA and PKG) on Ca21 mobilization were examined in dispersed smooth muscle cells. In permeabilized muscle cells, exogenous cAMP and cGMP inhibited in...