pes virus family and is transmitted via direct contact in all areas of world. In Japanese population, seropositivity for HCMV is higher. After primary infection, this virus causes latent infection in lineage-committed myeloid cells, including progenitors that give rise to granulocytes, macrophages, and dendritic cells. Pathogenesis of HCMV disease is directly linked to the immune status of the ...

The gross calorific value (GCV) or heating value of a sample of fuel is one of the important properties which defines the energy of the fuel. Many researchers have proposed empirical formulas for estimating GCV value of coal. There are some known methods like Bomb Calorimeter for determining the GCV in the laboratory. But these methods are cumbersome, costly and time consuming. In this paper, m...

Gene therapy has become a promising alternative treatment form for cancer. Among the broad range of different genetic means to reduce the tumor growth, herpes simplex virus thymidine kinase/ganciclovir (HSV-TK/GCV) suicide gene therapy regimen is the best known approach. In this type of therapy, cancer cells are manipulated to express HSV-TK, followed by administration of the prodrug, the antiv...

The causal relationship between neurogenesis and the recovery of poststroke cognitive function has not been properly explored. The current study aimed to determine whether depleting neuroprogenitor cells (NPCs) affects poststroke functional outcome in nestin-δ-HSV-TK-EGFP transgenic mice, in which the expression of a truncated viral thymidine kinase gene and EGFP was restricted to nestin-expres...

BACKGROUND The development of infections with ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) remains a serious problem in recipients of stem cell or organ transplants. Nearly all GCV-resistant clinical isolates have mutations in the viral UL97 gene. The rapid detection of GCV-resistant HCMV infections is necessary and the relative proportions of wild-type and mutant strains are predic...

BACKGROUND This study aimed to evaluate the effectiveness of gancyclovir (GCV) treatment for severe cytomegalovirus (CMV)-associated pneumonia in immunocompetent children. METHOD We enrolled patients with CMV-associated severe pneumonia admitted to the Vietnam National Hospital of Pediatrics, Hanoi, Vietnam, from January 2010 to December 2011. On admission, though respiratory bacteria and vir...

BACKGROUND In haematopoietic stem cell transplant (HSCT) recipients, cytomegalovirus (CMV) infection contributes significantly to morbidity and mortality in both the early and late post-transplant period. Ganciclovir (GCV) is the treatment of choice for CMV, but requires intravenous administration, a fact that complicates its long-term use. Oral valganciclovir (VGCV) and intravenous GCV were re...

The aim of the present study was to investigate the therapeutic potential of a double suicide gene, thymidine kinase (TK) combined with cytosine deaminase (CD), mediated by generation of 5-polyamidoamine dendrimers (G5-PAMAM-D) on human Tenon's capsule fibroblasts (HTFs) as an anti-scarring agent. The pAcGFP1-Hyg-TK-CD plasmid was transfected into HTFs, and reverse-transcription polymerase chai...

Ganciclovir (GCV) resistance is an emerging problem for transplant recipients. A sensitive and rapid real-time PCR approach for simultaneous and semiquantitative detection of human cytomegalovirus (HCMV) UL97 mutations in codons 460/520 was established by LightCycler and confirmed by restriction fragment length polymorphism and sequencing. Results from HCMV laboratory strains were compared with...

A nonlinear wavelet shrinkage estimator was proposed in Huang and Lu (2000). Such an estimator combined the asymptotic equivalence to the best linear unbiased prediction and the Bayesian estimation in nonparametric mixed-effects models. In this article a data-driven GCV method is proposed to select hyperparameters. The proposed GCV method has low computational cost and can be applied to one or ...