The aim of the present study was to investigate the molecular mechanism of carbapenem resistance of three imipenem-resistant isolates of Myroides odoratimimus recovered from two livestock farms of cows and pigeons by rectal swab in Lebanon in January 2014. Investigation of imipenem resistance of these isolates using the modified Hodge test, the EDTA test, the modified CarbaNP test and the matri...

ceftazidime, and imipenem (MIC, 132 mg/L); intermediate susceptibility to aztreonam; and susceptibility to piperacillin. Such a phenotype suggested MBL production, which was confirmed by the imipenem-EDTA double-disk synergy test [6]. The MBL determinant was subsequently identified as blaIMP-4 by molecular techniques [7]. The patient, who had just been transferred to the neurosurgery department...

BACKGROUND AND OBJECTIVES Acinetobacter causes a wide variety of illness in debilitated and hospitalized patients. Carbapenem resistance in Acinetobacter is an emerging problem and is a cause of concern as many nosocomial infections with Acinetobacter are resistant to most other antibiotics. The present study was aimed to study metallo-β-lactamase (MBL) production in Acinetobacter species. MA...

Metallo-β-lactamase (MBL) producing Pseudomonas aeruginosa has been reported to be an important nosocomial infection. Its intrinsic and acquired resistance to various antimicrobial agents and its ability to develop multidrug resistance imposes a serious therapeutic problem. Different clinical samples were collected from public and private hospitals in Baghdad city, Iraq. Bacterial identificatio...

BACKGROUND/AIM The aim of this study was to determine the molecular characteristics and antibiotic resistance of 13 (10 inpatient and three outpatient) Acinetobacter baumannii beta-lactamase-producing isolates collected in Bosnia and Herzegovina between December 2009 and May 2010. MATERIALS AND METHODS Susceptibility testing was performed by disk diffusion and broth microdilution methods. The...

with reduced susceptibility (,23 mm inhibition zone diameter or MIC .1 mg/L) to any of the carbapenems (ertapenem, imipenem or meropenem) will be tested for the carbapenemase phenotype by the combined disc method (with EDTA or boronic acid). All strains with a positive phenotype will be sent to a reference laboratory for molecular testing. Hence, implementation of the new CLSI criteria would no...

PURPOSE This study was undertaken to analyze the extended spectrum of β lactamase (ESBL), metallo β lactamase (MBL) & AmpC production in Pseudomonas aeruginosa in various clinical samples. MATERIALS & METHODS One hundred four non repetitive clinical specimens were inoculated onto nutrient agar, blood agar and incubated at 37(o)C overnight. The colonies were tested for oxidase test and other b...

BACKGROUND Acinetobacter spp. is an opportunistic pathogen that has demonstrated increasing relevance in nosocomial infections. Carbapenem-resistant strains have been reported worldwide. METHODS Since 2014, screening for metallo-β-lactamases (MBLs) in all Acinetobacter spp. isolates using phenotypic methods and PCR has been implemented at the University Hospital Center Zagreb. RESULTS The b...

background & objectives: pseudomonas aeruginosais one of the most important causes of nosocomial infections particularly in immunodeficient patients. several antibiotics such as betalactams, aminoglycosides and quinolones are used for the treatment of infections caused by this organism, but the prevalence of multidrug resistant strains has been reported frequently. beta lactamase production is ...