Urine was collected from newborns, children, and adults who had received a tracer dose of [3H] cortisol. The free and different types of conjugated cortisol metabolites were separated by ion-exchange chromatography. A modified Amberlite XAD-2 procedure was used, which enabled quantitative extraction of the extremely polar cortisol metabolites from urine from newborns. Large differences between ...

A new teicoplanin-like antibiotic was discovered when using Actinoplanes teichomyceticus strain 3/W, the fermentation medium containing Alburex, and the fermentation time 275 hours. The new product was separated from teicoplanin complex by polyamide resin chromatography and purified by Amberlite XAD-7 and affinity resin chromatographies. The structure was established on the basis of the physico...

Black peanut skins as a byproduct from peanut industry contain abundant anthocyanins, evaluated as 8.61±0.27mg/g dry black peanut skins, are currently poorly exploited. In this work, four anthocyanins and three major flavonols were detected and identified by HPLC-PDA-ESI-MS/MS from the acidified water extract of black peanut skins of Arachis hypogaea L. After preliminary removal of flavonols by...

Phenolic compounds were extracted from defatted flaxseeds using ethanol-dioxane (1:1, v/v). The crude extract obtained was purified using Amberlite XAD-16 column chromatography with water and methanol as mobile phases. RP-HPLC and SE-HPLC showed a lignan macromolecule (LM) as a dominant phenolic compound in the purified extract. After the alkaline hydrolysis of LM caffeic acid glucoside (CaAG) ...

In a group of dogs in whom fulminant liver failure had been induced, perfusion of blood through activated charcoal resulted in a significantly longer survival than that of a similar group of dogs whose blood was not so treated. An otherwise progressive rise in blood ammonia concentration was halted in the treatment group. In another group of dogs with fulminant liver failure perfusion of blood ...

After the growth of Paecilomyces persicinus P-10 in a glucose-peptone medium, filtrates were collected and analyzed for antibiotic antivity. Activities against Salmonella gallinarum ATCC 3030 and Alcaligenes faecalis ATCC 8750 (penicillin N-resistant strain) were obtained. Part of the former activity was readily inactivated by penicillinase. The fraction active against A. faecalis was isolated ...

This paper presents the development of two optosensors for the determination of four polycyclic aromatic hydrocarbons (anthracene, benzo[a]pyrene, fluoranthene and benzo[b]fluoranthene) using a photomultiplier device and an intensified coupled charge device (ICCD) as optical transducers, respectively. These optosensors are based on the on-line immobilization of the analytes onto a non-ionic res...

A simple, convenient method is described for the detection in human urine of three major classes of drugs of abuse: amphetamines, barbiturates, and alkaloids. The drugs are adsorbed from urine by a column of non-ionic resin (Amberlite XAD.2), eluted with methanol, and chromatographed on a thinlayer silica gel plate. The drugs are made visible and identified by a series of procedures involving e...

Vitamin B12, Cobyric Acid, Fe(III)Cobalamin, Fe(III)Cobyric Acid, Iron-B12-Analogues, UV/VIS-, NMR-, ESR-Spectra The reaction of hydrogenocobalamin or hydrogenocobyric acid (both isolated from Chromatium vinosum) with iron(III) acetate and iron powder in acetic acid under N 2 at 80 °C leads to the incorporation of iron into these cobalt-free corrinoids. The products, Fe(III)cobalamin and Fe(ni)...