In vitro assembled yeast ribosome-nascent chain complexes (RNCs) containing a signal sequence in the nascent chain were immunopurified and reconstituted with the purified protein-conducting channel (PCC) of yeast endoplasmic reticulum, the Sec61 complex. A cryo-EM reconstruction of the RNC-Sec61 complex at 15.4 A resolution shows a tRNA in the P site. Distinct rRNA elements and proteins of the ...

This paper presents a new algorithm for nding global min-cuts in weighted, undirected graphs. One of the strengths of the algorithm is its extreme simplicity. This randomized algorithm can be implemented as a strongly polynomial sequential algorithm with running time ~ O(mn), even if space is restricted to O(n), or can be parallelized as an RNC algorithm which runs in time O(log n) on a CRCW PR...

The type I signal peptidase lepB genes from Rickettsia rickettsii and Rickettsia typhi, the etiologic agents of Rocky Mountain spotted fever and murine typhus, respectively, were cloned and characterized. Sequence analysis of the cloned lepB genes from R. rickettsii and R. typhi shows open reading frames of 801 and 795 nucleotides, respectively. Alignment analysis of the deduced amino acid sequ...

Salmonella typhimurium LT2 contains intervening sequences (IVSs) of 90-110 nt within all its 23S rRNA that are cleaved out by RNase III, resulting in rRNA fragmentation. In order to determine the functionality of 23S rRNA that contains unexcised IVSs, we constructed an S. typhimurium RNase III (rnc) deficient strain by transducing a mini-Tn10 (rnc-14::Tn10) from Escherichia coli K-12. The resul...

A minimum cut is a set of edges of minimum weight whose removal disconnects a given graph. Minimum cut algorithms historically applied duality with maximum ows and thus had the same (mn) running time as maximum ow algorithms. More recent algorithms which are not based on maximum ows also require (mn) time. In this paper, we present the rst algorithm that breaks the (mn) \max-ow barrier" for ndi...

Green home rating has emerged as an important agenda to practice the principles of sustainability. In Malaysia, the establishment of the ‘Green Building Index – Residential New Construction’ (GBI-RNC) has brought this agenda closer to the stakeholders of the local green building industry. GBI-RNC focuses on the evaluation of the environmental impacts posed by houses rather than assessing the Tr...

The combination of perfective aspect and present tense is frequently considered as an example semantically incompatible grams. If verbal forms including markers both do exist in a language, they tend not to express resp. the strict sense. Thus, Russian such usually convey future, napishu ‘I will write’. article discusses specific type contexts where these develop less trivial meaning what can b...

Follow this and additional works at: http://opensiuc.lib.siu.edu/math_articles This is a preprint (prior to peer review) of the article published as Tall, I. A., & Respondek, W. (2009). Analytic normal forms and symmetries of strict feedforward control systems. International Journal of Robust and Nonlinear Control. doi: 10.1002/rnc.1505. The definitive version is available at http://onlinelibra...