نتایج جستجو برای: تکنیک lamp

تعداد نتایج: 37531  

ژورنال: دنیای میکروب ها 2012

روش تکثیر هم دمایی به واسطه حلقه (LAMP) مبتنی بر تکثیر اسید نوکلئیک در دمای ثابت ˚C 65-62 است.  در این روش بدون نیاز به مرحله واسرشت شدن حرارتی، به کمک آنزیم DNA پلی مراز Bst به صورت هم زمان امکان جداسازی و تکثیر دو رشته DNA وجود دارد. بنابراین بر خلاف سایر روش های متداول تکثیری ، بدون نیاز به چرخه های دمایی و دستگاه ترموسایکلر و با استفاده از تجهیزات ارزان قیمتی مانند حمام آب گرم و یا...

باکویی کتریمی, علی, حسینی, سید عبدالله, دریانی, احمد, سروی, شهاب الدین, شریف, مهدی, عمویی, افسانه, میزانی, آزاده, گیل, پوریا,

Background and purpose: Fasciola hepatica and Fasciola gigantica are common liver flukes which affect both human and livestock worldwide. In this study we evaluated the loop-mediated isothermal amplification (LAMP) assay for detection and discrimination of Fasciola species. Materials and methods: Fifty adults of Fasciola worms were isolated from sheep and cattle liver form abattoirs in Mazand...

Journal: :Journal of cardiology 2015
Yoko Hashida Taizo Wada Takekatsu Saito Kunio Ohta Yoshihito Kasahara Akihiro Yachie

INTRODUCTION Danon disease is an extremely rare X-linked dominant disorder characterized by progressive cardiomyopathy, muscle weakness, and mild mental retardation. Most cases harbor nonsense, frameshift, or splice-site mutations in LAMP2 that result in lysosome-associated membrane protein-2 (LAMP-2) deficiency and lysosomal defects. The identification of LAMP2 mutations makes it possible to d...

Journal: :European journal of orthodontics 2004
A P Pettemerides M Sherriff A J Ireland

The purpose of this study was to compare the effectiveness of a plasma arc lamp with a conventional tungsten quartz halogen lamp in orthodontic bonding. Twenty consecutive patients had their brackets bonded either with Transbond XT (n = 10) or Fuji Ortho LC (n = 10). In total, 352 teeth were bonded, 176 in each group. Using a randomized cross-mouth control study design, where diagonally opposit...

2004
Allen R. Hanson

A compact visual representation, called the 3D layered, adaptive-resolution, and multiperspective panorama (LAMP), is proposed for representing large-scale 3D scenes with large variations of depths and obvious occlusions. Two kinds of 3D LAMP representations are proposed: the relief-like LAMP and the image-based LAMP. Both types of LAMPs concisely represent almost all the information from a lon...

2016
Yumiko HIGA Ryoko UEMURA Wataru YAMAZAKI Shinya GOTO Yoshitaka GOTO Masuo SUEYOSHI

We improved a loop-mediated isothermal amplification (LAMP) assay permitting sensitive and rapid Mycoplasma bovis detection. A total of 55 bacterial strains were examined in this study, including 33 M. bovis strains, 14 non-M. bovis mycoplasmas and eight non-mycoplasma bacterial strains. M. bovis was successfully detected by the LAMP assay within 60 min without cross-reaction to any other bacte...

2006
M. Doshi Montu Doshi Regan Zane Francisco J. Azcondo

This paper presents a low-frequency square-wave drive, consisting of a single high voltage converter, an ac lamp ignition circuit, current control devices and a single backlight controller, capable of driving an arbitrary number of parallel cold cathode fluorescent lamps (CCFLs) with independent accurate lamp current regulation. Key to the architecture is a proposed capacitive coupling approach...

2013
Yanhua Wang Guangxiang Wang Delin Zhang Hong Yin Meng Wang

A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In inf...

2013
Jing Liu Liping Xu Jinlong Guo Rukai Chen Michael Paul Grisham Youxiong Que

Ratoon stunt, caused by the xylem-limited coryneform bacterium Leifsonia xyli subsp. xyli (Lxx), is a deep bacteriosis and prevalent in most of sugarcane-producing countries. Based on loop-mediated isothermal amplification (LAMP), we developed a method for detecting Lxx. The major advantages of the LAMP method are visual judgment by color and time saving with only 60 min for identification of L...

2016
Xulong Wu Lu Xiao Yin Wang Xueping Yao Bin Peng

A loop-mediated isothermal amplification (LAMP) assay was developed for rapid, sensitive and specific detection of African swine fever virus (ASFV). A set of LAMP primers was designed based on the sequence of the ASFV gene K205R. Reaction temperature and time were optimized to 64 o C and 60 min, respectively. LAMP products were detected by agarose gel electrophoresis or visually with the additi...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید