Isolation and cultivation are a crucial step in elucidating the physiology, biogeochemistry, and ecosystem role of microorganisms. Many abundant marine bacteria, including the widespread Roseobacter clade-affiliated (RCA) cluster group, have not been cultured with traditional methods. Using novel techniques of cocultivation with algal cultures, we have accomplished successful isolation and prop...

Rolling circle amplification (RCA) generates single-stranded DNAs or RNA, and the diverse applications of this isothermal technique range from the sensitive detection of nucleic acids to analysis of single nucleotide polymorphisms. Microwave chemistry is widely applied to increase reaction rate as well as product yield and purity. The objectives of the present research were to apply microwave h...

BACKGROUND/AIMS Continuous venovenous hemofiltration (CVVH) requires anticoagulation to prevent circuit clotting and its use is contraindicated in patients with high bleeding risk. The aim of this study was to compare CVVH with and without regional citrate anticoagulation (RCA) with respect to filter life, azotemic control and cost. METHODS This was a prospective sequential cohort study. The ...

Kleinbongard P, Baars T, Möhlenkamp S, Kahlert P, Erbel R, Heusch G. Aspirate from human stented native coronary arteries vs. saphenous vein grafts: more endothelin but less particulate debris. Am J Physiol Heart Circ Physiol 305: H1222–H1229, 2013. First published August 9, 2013; doi:10.1152/ajpheart.00358.2013.—Stent implantation into atherosclerotic coronary arteries releases particulate deb...

BACKGROUND A number of researchers have used different electrocardiographical criteria to predict the culprit vessel in acute interior wall myocardial infarction (MI) cases. Therefore, the determination of infarct related artery in AMI is extremely important with regard to prediction of potential complications, furthermole, predicting the probable site of occlusion within RCA is worthwhile beca...

BACKGROUND Recent studies performed with positron emission tomography have suggested that coronary flow reserve (CFR) is moderately to severely reduced after the arterial switch operation (ASO). These findings are of great concern but have not been confirmed by other methods. METHODS AND RESULTS Eleven symptom-free children were studied between 4 and 11 (median 6.0) years after the ASO. Flow ...

Using recycled concrete aggregate (RCA) as base/subbase for road construction is alternative way to manage construction waste and reduces the need for virgin aggregates. This paper discusses the long-term performance of concrete pavements on RCA granular layer in comparison to concrete pavements on virgin aggregate granular layer. 18 representative RCA pavement sections across Iowa were primary...

Microemulsions with limited stability in mimetic gastrointestinal environments have previously demonstrated potential for the effective removal of ammonia from artificial colonic fluid. Specialized pH‑sensitive microemulsion‑based gels for the removal of colonic ammonia (MBG‑RCA), however, possess relative stability in the gastrointestinal (GI) tract of normal rats, indicating potential use in ...

In patients with occlusion of the left anterior descending coronary artery (LAD) or right coronary artery (RCA), the conus artery, which arises at or near the origin of the RCA, often serves as a principal source of collateral circulation. Coronary arteriograms in 508 adult patients revealed that in 80.5% the conus artery was well visualized on the RCA angiogram, but that in 19.5% it was not ad...

While microarrays hold considerable promise in large-scale biology on account of their massively parallel analytical nature, there is a need for compatible signal amplification procedures to increase sensitivity without loss of multiplexing. Rolling circle amplification (RCA) is a molecular amplification method with the unique property of product localization. This report describes the applicat...