background and aims: canine distemper (cd) is a deadly infectious disease of canidae family. cd is a multi-systemic viral disease and is specified by wide range of clinical symptoms. the manifestations are not always indicative of cd, therefore a laboratory confirmation is necessary for suspected cases. materials and methods: different clinical specimens of 19 cd suspected unvaccinated dogs wer...

Q fever is a worldwide zoonosis caused by Coxiella burnetii-a small obligate intracellular Gram-negative bacterium found in a variety of animals. It is transmitted to humans by inhalation of contaminated aerosols from urine, feces, milk, amniotic fluid, placenta, abortion products, wool, and rarely by ingestion of raw milk from infected animals. Nested PCR can improve the sensitivity and specif...

The herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2) and varicella-zoster virus (VZV) can cause life-threatening infections of the central nervous system and lead to severe infections in immunocompromised subjects and newborns. In these cases, rapid diagnosis is crucial. We developed three different real-time PCR assays based on TaqMan chemistry for the LightCycler instrument to detect HS...

In the present study sensitivity of diagnosis of rabies with Nested RT-PCR was compared with Immunofluorescence on 20 brain samples. RNA extraction was done using Qiazol method. Synthesis of cDNA was done using rabies specific primers. Nested set of primers were used to amplify highly conserved 762bp nucleoprotein gene region. Nested RT-PCR was able to diagnose rabies viral RNA in 12 out of 13 ...

background: toxoplasmosis is a worldwide spread disease. the present study examined the prevalence of toxoplasma gondii infection among animals of ediblemeat (cattle and sheep) in chaharmahal va bakhtiari province (southwest of iran) in 2012. furthermore, we attempted for the first time to identify this parasite from the meat products in the province. methods: the tongue, brain, femur muscle an...

background: the incidence of cutaneous leishmaniasis in the city of mehran has risen sharply in recent years because the city borders iraq, which has allowed entrance of different leishmania strains. these strains have different shapes, periods of disease, and healing of lesions. the present study identified and determined cutaneous leishmaniasis species in this region. methods : this cross-sec...

conclusions this is the first molecular report on parasite infection of both vector (p. papatas) and reservoir (t. indica) to l. major from this area. therefore, the population of both sandflies and rodents should be reduced using control methods. background leishmaniais which is one of the six most important tropical diseases in the world are in iran in 3 forms. cutaneous leishmaniasis (cl) is...