Background. How host-symbiont interactions coevolve between mutualism and parasitism depends on the ecology of the system and on the genetic and physiological constraints of the organisms involved. Theory often predicts that greater reliance on horizontal transmission favors increased costs of infection and may result in more virulent parasites or less beneficial mutualists. We set out to under...

Gene 5 protein (gp5) of bacteriophage T7 is a non-processive DNA polymerase, which acquires high processivity by binding to Escherichia coli thioredoxin. The gene 5 protein-thioredoxin complex (gp5/trx) polymerizes thousands of nucleotides before dissociating from a primer-template. We have engineered a disulfide linkage between the gene 5 protein and thioredoxin within the binding surface of t...

Conditional lethal YidC mutants have been isolated to decipher the role of YidC in the assembly of Sec-dependent and Sec-independent membrane proteins. We now show that the membrane insertion of the Sec-independent M13 procoat-lep protein is inhibited in a short time in a temperature-sensitive mutant when shifted to the nonpermissive temperature. This provides an additional line of evidence tha...

An analysis of the CN and CH band strengths measured in a large sample of M71 and M13 main sequence stars by Cohen (1999a,b) is undertaken using synthetic spectra to quantify the underlying C and N abundances. In the case of M71 it is found that the observed CN and CH band strengths are best matched by the identical C/N/O abundances which fit the bright giants, implying: 1) little if any mixing...

The construction of recombinant M13 phages containing adenovirus DNA inserts was undertaken to provide strand-specific hybridization probes for analyses of adenovirus type 2 RNA transcripts. A library of molecular probes was constructed by cloning restriction endonuclease fragments of adenovirus types 2 and 5 DNA in the duplex replicative form DNA of the single-stranded bacteriophage vectors, M...

Geobacillus stearothermophilus is the main thermophilic spore former involved in flat sour spoilage of canned foods. Three typing methods were tested and applied to differentiate strains at intra-species level: panC sequence analysis, REP-PCR and M13-PCR. panC gene was highly conserved within the studied strains, suggesting a low intra-specific diversity. This was supported by REP-PCR primary a...

4 Tarek M. Gibreel, Andrew R. Dodgson, John Cheesbrough, Frederick J. Bolton, 5 Andrew J. Fox and Mathew Upton 6 7 8 Microbiology and Virology Unit, School of Medicine, University of Manchester, Stopford 9 Building, Oxford Road, Manchester, M13 9PL, United Kingdom. Central Manchester 10 Foundation Trust, Oxford Road Manchester, Manchester, M13 9WL. Preston Microbiology 11 Services, Royal Presto...

MONSTR Rule Systems R. Banach Computer Science Dept., Manchester University, Manchester, M13 9PL, U.K.

Analyses of genetic diversity within populations could be of great benefit to plant genetic resources conservation . In order to identify genetic markers that are variable within populations, the genome of Phaseolus vulgaris was screened with several DNA sequences in order to identify hypervariable sequences . Polymorphisms were observed between Middle American and Andean cultivars using the pr...

BACKGROUND Cytochrome P450 monooxygenase constitutes a significant group of oxidative enzymes that can introduce an oxygen atom in a high regio- and stereo-selectivity mode. We used the Bacillus megaterium cytochrome P450 BM3 (CYP450 BM3) and its variants namely mutant 13 (M13) and mutant 15 (M15) for the hydroxylation of diverse class of flavonoids. RESULTS Among 20 flavonoids, maximum seven...