National Institute of Health and Nutrition, 1–23–1 Toyama, Shinjuku-ku, Tokyo 162–8636, Japan, Sendai Shirayuri Women’s College, 6–1 Honda-cho, Izumi-ku, Sendai city, Miyagi 981–3107, Japan, Otsuma Women’s University, 12 Sanban-cho, Chiyoda-ku, Tokyo 102–8357, Japan, University of Tokyo, 7–3–1 Hongo, Bunkyo-ku, Tokyo 113–8656, Japan, and University of Tsukuba, 1–1–1 Tennodai, Tsukuba city, Ibar...

Taizo Yamada, Kenro Aihara, Noriko Kando, Satoko Fujisawa, Yusuke Uehara, Takayuki Baba, Shigemi Nagata, Takashi Tojo, Yuko Hiroshima and Jun Adachi 1 National Institute of Informatics, 2-1-2 Hitotsubashi, Chiyoda-ku, Tokyo, Japan 2 Dept. of Informatics, the Graduate University for Advanced Studies, 2-1-2 Hitotsubashi, Chiyoda-ku, Tokyo, Japan 3 Fujitsu Laboratories Ltd., 1-1 Kamikodanaka 4, Na...

1 Cray Japan, Inc., 41-12 Nihonbashi, Hakozaki-cho, Chuo-ku, Tokyo 103-0015, Japan 2 Graduate School of Mathematics, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan 3 Protonic NanoMachine Project, ERATO, JST, 1-7 Hikaridai, Seika, Souraku, Kyoto 619-0237, Japan 4 RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan 5 National Institut...

1 Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, O&N 1, Herestraat 49 PB813, B-3000 Leuven, Belgium 2 Dept. Development and Regeneration Skeletal Biology and Engineering Research Center, KU Leuven, O&N 1, Herestraat 49 PB813, B-3000 Leuven, Belgium 3 Dept. Chemistry Molecular Design and Synthesis, KU Leuven, Celestijnenlaan 200f – PB2404, B-3001 Leuven, Belgium 4 Dept. Clinical...

Telomere length maintenance, an activity essential for chromosome stability and genome integrity, is regulated by telomerase- and telomere-associated factors. The DNA repair protein Ku (a heterodimer of Ku70 and Ku80 subunits) associates with mammalian telomeres and contributes to telomere maintenance. Here, we analyzed the physical association of Ku with human telomerase both in vivo and in vi...

In Saccharomyces cerevisiae, the Ku heterodimer contributes to telomere maintenance as a component of telomeric chromatin and as an accessory subunit of telomerase. How Ku binding to double-stranded DNA (dsDNA) and to telomerase RNA (TLC1) promotes Ku's telomeric functions is incompletely understood. We demonstrate that deletions designed to constrict the DNA-binding ring of Ku80 disrupt nonhom...

We have characterized the biochemical nature of the Ku protein, the antigen recognized by autoantibodies from certain patients with scleroderma-polymyositis overlap syndrome. From extracts of HeLa cells labeled with [32P]orthophosphate, anti-Ku antibodies precipitated a high molecular weight nucleic acid identified as DNA because of sensitivity to DNase I and resistance to RNase. From extracts ...

Ku, a cellular complex required for human cell survival and involved in double strand break DNA repair and multiple other cellular processes, may modulate retroviral multiplication, although the precise mechanism through which it acts is still controversial. Recently, Ku was identified as a possible anti-human immunodeficiency virus type 1 (HIV-1) target in human cells, in two global approaches...

Methods and results Prick-prick carried out with flaxseed showed a strong positivity. Skin prick test to inhaled allergens were positive to mites, dog and cat danders, plain tree and grasses. Skin prick test to food allergens were positive to almond, hazelnut, peanut, chestnut, walnut, mustard, lentils, soybeans, rice, oat, corn and lupine. Total IgE was: 291 UI/ml. Specific IgE (CAP, ThermoFis...