Competence is a physiological state that enables Bacillus subtilis 168 to take up and internalize extracellular DNA. In practice, only a small subpopulation of B. subtilis 168 cells becomes competent when they enter stationary phase. In this study, we developed a new transformation method to improve the transformation efficiency of B. subtilis 168, specially in rich media. At first, different c...

Department of Management Information System, National Chung Hsing University, 250, Kuo Kuang Rd., Taichung, 413 Taiwan, ROC Department of Information Management, Chaoyang University of Technology, 168, Gifeng E. Rd., Wufeng, Taichung County, 413 Taiwan, ROC Graduate Institute of Networking and Communication Engineering, Chaoyang University of Technology, 168, Gifeng E. Rd., Wufeng, Taichung Cou...

The expression of osteopontin (OPN) in boar testis was studied. Western blot analysis detected 66- and 32-kDa OPN immunopositive bands in the testes of adult boars. In postnatal piglets, the 66-kDa OPN band was detected in the testes, but not the 32-kDa band. In the newborn testis, OPN immunostaining was seen in gonocytes and in some supporting cells in the seminiferous tubules, as well as in i...

ISSN: 1933-6950 (Print) 1933-6969 (Online) Journal homepage: http://www.tandfonline.com/loi/kchl20 Ryanodine receptors Mounia Chami & Frédéric Checler To cite this article: Mounia Chami & Frédéric Checler (2014) Ryanodine receptors, Channels, 8:3, 168-168, DOI: 10.4161/chan.29000 To link to this article: http://dx.doi.org/10.4161/chan.29000 Copyright © 2014 Landes Bioscience Published online: 2...

BACKGROUND Poliovirus types 1 and 3 co-circulate in poliomyelitis-endemic countries. We aimed to assess the immunogenicity of a novel bivalent types 1 and 3 oral poliovirus vaccine (bOPV). METHODS We did a randomised, double-blind, controlled trial to assess the superiority of monovalent type 2 OPV (mOPV2), mOPV3, or bOPV over trivalent OPV (tOPV), and the non-inferiority of bivalent vaccine ...

Background-Patients with cancer have long periods of severe neutropenia due to chemotherapy and are particularly vulnerable to infectious complications. Most fever episodes are treated with empiric antibacterial therapy without identifying the etiologic agent. Blood culture is the gold standard test to detect infections, but it usually presents negative results. Human herpes virus establishes l...

Microcentrifuge tubes containing 8 x 10(6) purified oocysts of Cryptosporidium parvum suspended in 400 microliters of deionized water were stored at 5 degrees C for 168 h or frozen at -10, -15, -20, and -70 degrees C for 1 h to 168 h and then thawed at room temperature (21 degrees C). Fifty microliters containing 10(6) oocysts was administered to each of five to seven neonatal BALB/c mice by ga...

The synthesis of the Ethernet is an appropriate riddle. In fact, few systems engineers would disagree with the visualization of hierarchical databases [114, 188, 114, 62, 70, 179, 188, 68, 95, 54, 152, 191, 59, 168, 148, 99, 58, 129, 168, 128]. We use omniscient methodologies to verify that context-free grammar and writeahead logging can synchronize to answer this quandary.