نتایج جستجو برای: آنزیم pfu
تعداد نتایج: 13009 فیلتر نتایج به سال:
The SARS-CoV-2 pandemic caused a public health crisis throughout the world and highlighted need for rapid sensitive testing as countermeasure. A specific biosensor platform is developed detection of antigen RNA SARS-CoV-2, its variant (B1.1.529). demonstrated combines unique protein catalyzed capture bioreceptors (PCCs) chimeric (RNA-DNA) probe using LwaCas13a collateral cleavage activity atop ...
هدف از این تحقیق، ارزیابی فرآیند حرارتی مورد استفاده، تاثیر زمان نگهداری و اثر محتوی چربی ماست تولیدی بر بقای ویروس male-specific coliphage (ms2) بود. میزان چربی شیر در سه سطح (٥/۱%، ٥/٢% و ٣%)، میزان تلقیح ویروس در سه سطح pfu/ml)107 و 105 و 103) و زمان ماندگاری ماست در ده سطح (ده روز متوالی) بعنوان تیمارهای مورد بررسی انتخاب شدند. آزمون های مورد بررسی به منظور ارزیابی بقای انتروباکتریوفاژ ms2،...
SRC kinases and PKR are intracellular protein kinases, which play key roles in intracellular viral replication. In this research, the effect of SRC kinase inhibition and PKR activation and inhibition on replication of coxsakievirus (CVB3), an entrovirus of the family picornaviridae – causative agents of fatal myocarditis, was studied. Vero and Hela cells were cultured and infected with CVB3 in ...
In this study, three types of treated wastewater were tested for infectious enteroviruses, the enterovirus genome, somatic coliphages, and Bacteroides fragilis phages. The aim of this work was to determine whether the presence of the two types of bacteriophages or of the enterovirus genome was a good indicator of infectious enterovirus contamination. The enterovirus genome was detected by rever...
The use of bacteriophages in the treatment and prevention of infections by the fish pathogen Flavobacterium psychrophilum has attracted increased attention in recent years. It has been shown recently that phage delivery via the parenteral route resulted in immediate distribution of phages to the circulatory system and the different organs. However, little is known about phage dispersal and surv...
Coliphage content of sewage collected from 11 different localities in Hong Kong was determined. The number of plaque-forming units (PFU) ranged from 0.036 x 10(3) to 15.9 x 10(3) per ml. In general, urban sewage tended to be richer than rural sewage both in PFU count as well as plaque morphological variation. Seventy-seven isolates were subjected to a host range study. Fifty per cent of these w...
BACKGROUND We conducted a Phase I randomized, dose-escalation, route-comparison trial of MVA-CMDR, a candidate HIV-1 vaccine based on a recombinant modified vaccinia Ankara viral vector expressing HIV-1 genes env/gag/pol. The HIV sequences were derived from circulating recombinant form CRF01_AE, which predominates in Thailand. The objective was to evaluate safety and immunogenicity of MVA-CMDR ...
گیاهان دارای دو دسته متابولیت های اولیه و ثانویه می باشند. متابولیت های اولیه به صورت مستقیم در رشد و نمو تاثیر می گذارند، در حالی که متابولیت های ثانویه به صورت عمومی نبوده و به طور مستقیم بر رشد و نمو تاثیر نمی گذارند. این ترکیبات دارای طیف گسترده و متنوعی در گیاهان بوده و برخی از آن ها متعلق به خانواده خاصی از موجودات می باشند. برای مثال، ایزوفلاونوییدها دسته ای از ترکیبات فلاونوییدی هستند ک...
The effects of centrifugation of the infection of cell culture with bluetongue virus (BTV) were investigated. Baby hamster kidney cells were infected with BTV with or without centrifugation. Viral antigen was detected by immunofluorescence at 24 h in both centrifuged and noncentrifuged cultures. However, after 24 h of infection, the production of PFU in centrifuged cell cultures was 10- to 20-f...
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