In situ biosurfactant (rhamnolipid) production by Pseudomonas putida CB-100 was achieved during a bioaugmented and biostimulated treatment to remove hydrocarbons from aged contaminated soil from oil well drilling operations. Rhamnolipid production and contaminant removal were determined for several treatments of irradiated and non-irradiated soils: nutrient addition (nitrogen and phosphorus), P...

Vegetative plants of certain seaweeds were collected from the natural habitat of Kilakarai and Mandapam coast were subjected to SEM-energy dispersive spectroscopic analysis and quantified the following minerals viz., Na, Mg, Si, S, Cl, K, Ca, Mn, P, Fe, Zn and Cr during summer, pre-monsoon, monsoon and post-monsoon seasons in 2007-2008. The order of preferential accumulation of elemental compos...

This study addresses the role of ATP-bound and free Mg and Mn ions in the activation and modulation of chaperonin-assisted refolding of urea-denatured malate dehydrogenase. As compared with Mg, Mn ions caused a significant increase in the rate of GroE-assisted malate dehydrogenase refolding and, concomitantly, a decrease in the rate of ATP hydrolysis. Moreover, Mn increases the affinity of GroE...

Impacts of metals to the growth and enzyme production of fungi were tested with Cd(0-10 mg kg–1), Co(0-100 mg kg–1), Cr(0-100 mg kg–1), Li(0-100 mg kg–1) or Mn(0-400 mg kg–1) containing media with ABTS [2,2’-azino-bis (3-ethylbentzthiazoline6-sulfonicacid)] as indicator of oxidative enzymes, laccase and/ or peroxidases. All ascomycetes Alternaria sp., Chaetomium sp., Epicoccum sp., Fusarium sp....

Skin explants were placed in plastic dishes coated with fibronectin (FN), fibrinogen (FGN) or collagen. Explants were cultured for 16 h in serum-free medium containing calcium (Ca) and magnesium (Mg), or in medium containing either Ca-only, Mg-only or manganese (Mn)-only. In Ca/Mg, migrating keratinocytes on all test substrata produced a sheet of contiguous cells that formed a robust halo aroun...