PURPOSE Disruption of widely expressed essential genes in mice often leads to embryonic or neonatal lethality. To circumvent this problem and dissect gene functions in the cone photoreceptors, we elected to generate cone photoreceptor specific cre transgenic mice. METHODS Transgenic mice expressing Cre recombinase directed by the human red/green pigment (HRGP) gene promoter were generated. Ca...

Biological discovery in the postgenomic era requires a systematic and high-throughput experimental approach. To this end, a versatile PCR-based tandem epitope tagging system is described, which inserts a tandem epitope coding sequence into any desired position of the Escherichia coli chromosome. Template plasmids were constructed that carry tandem copies of the epitope encoding sequence, Flp re...

We study the renormalization group evolution and infra-red stable fixed points of the Yukawa couplings and the soft supersymmetry breaking trilinear couplings of the minimal supersymmetric standard model with baryon and lepton number (and R-parity) violation involving the heaviest generations. We show analytically that in the Yukawa sector there is only one infra-red stable fixed point. This co...

The FLP enzyme catalyzes recombination between specific target sequences in DNA. Here we use FLP to temporally and spatially control gene expression in the nematode C. elegans. Transcription is blocked by the presence of an "off cassette" between the promoter and the coding region of the desired product. The "off cassette" is composed of a transcriptional terminator flanked by FLP recognition t...

In this paper, we will obtain first integral, integrating factor and λ-symmetry of third-order ODEs u ⃛=F(x,u,u ̇,u ̈). Also we compare Prelle -Singer (PS) method and λ-symmetry method for third-order differential equations.In this paper, we will obtain first integral, integrating factor and λ-symmetry of third-order ODEs u ⃛=F(x,u,u ̇,u ̈). Also we compare Prelle -Singer (PS) method and λ-symmetry m...

BACKGROUND Escherichia coli K-12 is a frequently used host for a number of synthetic biology and biotechnology applications and chassis for the development of the minimal cell factories. Novel approaches for integrating high molecular weight DNA into the E. coli chromosome would therefore greatly facilitate engineering efforts in this bacterium. RESULTS We developed a reliable and flexible la...

In September 1998 the workshop for “Conditional Genome Alterations” in Cold Spring Harbor had a simple but important take-home message: “Cre works” (Rossant and McMahon, 1999). After several years of careful investigations, geneticists are now certain that the Cre recombinase of the P1 bacteriophage efficiently catalyzes recombination between two of its consensus 34 base pair DNA recognition si...

Androgens are key drivers of spermatogenesis, and germ cells in mice lacking androgen receptor (AR), specifically from Sertoli cells, arrest in meiosis (reviewed in Smith and Walker, 2014). When Sertoli-cell AR is ablated during fetal life (De Gendt and Verhoeven, 2012), however, it is impossible to determine whether the meiotic-arrest phenotype observed in adults results from perturbed Sertoli...