Human carbonic anhydrase B loses enzymatic activity as it undergoes an irreversible conformational change near pH 4 (1). Riddiford (2) further found that seven of the eleven histidyl groups, which are unreactive in the native protein, become titratable during this change. Also, five or six of the eight tyrosyl groups do not ionize freely and reversibly in the native protein (2, 3). Human carbon...

The morphological and cytochemical changes in erythroid-cell differentiation have been extensively studied: application of the velocity-sedimentation technique has provided preliminary data on the associated biochemical changes (Denton & Arnstein, 1973). After the last cell division there is a dramatic increase in the synthesis of haemoglobin which, at least in part, probably accounts for the a...

Combinatorial small molecule growth algorithm was used to design inhibitors for human carbonic anhydrase II. Two enantiomeric candidate molecules were predicted to bind with high potency (with R isomer binding stronger than S), but in two distinct conformations. The experiments verified that computational predictions concerning the binding affinities and the binding modes were correct for both ...

Davenport and Fisher (1938) reported the discovery DOGS P School of Medicine of large amounts of carbonic anhydrase in the gastric mucosa of cats, rats and rabbits. Davenport (1939) demonstrated that carbonic anhydrase is present in high concentration in the parietal cells and in lower concentration in the cells of the surface epithelium of the gastric mucosa of cats and rats. When those invest...

The carbonic anhydrase (CA: Carbonate hydrolyase: E.C.4.2.1.1) content from the leaves and flowers of mature yarrow (Achillea millefolium), a plant believed to possess healing qualities, was purified and characterized. The purification levels were 35.58 fold in the leaves and 40.21 fold in the flowers. The optimum temperatures were 25C and 40C for the leaves and flowers, respectively, and optim...

The distribution and biochemical diversity of neuroglial cells in the avian optic tectum were analyzed using immunohistochemical techniques. Polyclonal and monoclonal antibodies were employed to define the distributions and localizations of four proteins that are considered to be glial-specific in the mammalian central nervous system: glutamine synthetase, carbonic anhydrase-C, glial fibrillary...