In mammals, Toll-IL-1R-containing adaptor molecule 1 (TICAM1)-dependent TLR pathways induce NF-kappaB and IFN-beta responses. TICAM1 activates NF-kappaB through two different pathways involving its interactions with TNFR-associated factor 6 and receptor-interacting protein 1. It also activates IFN regulatory factor 3/7 through its interaction with TANK-binding kinase-1, leading to the robust up...

The antiviral immune responses were triggered by the innate immune recognition of viral infection. The type I IFNs (IFN-beta and IFN-alpha) are the key cytokines produced upon viral infection and consequently link innate immunity with adaptive immunity. A main antiviral system in mammals is TRIF-dependent TLRs pathway, but the TRIF-independent RIG-I pathway, has also been discovered recently. I...

When fish live in the wild or are cultured artificially, they will inevitably suffer from hypoxia. At same time, blood physiological indexes represent state of fish. In order to study effect long-term hypoxia acclimation on hematogenesis, we zebrafish embryos into adulthood a incubator (1.5 ± 0.2 mg/L). Then compared hematological parameters normoxia and conditions. Transcriptome sequencing ana...

Microcystin-LR (MC-LR) and glyphosate (GLY) have been classified as a Group 2B 2A carcinogens for humans, respectively, frequently found in aquatic ecosystems. However, data on the potential hazard of MC-LR GLY exposure to fish gut are relatively scarce. In current study, subacute toxicity test zebrafish exposed (35 μg L−1) (3.5 mg L−1), either alone or combination was performed 21 d. The resul...

KEY POINTS Zebrafish provide a unique opportunity to investigate in vivo sensory transduction in mature hair cells. We have developed a method for studying the biophysical properties of mature hair cells from the lateral line of juvenile zebrafish. The method involves application of the anaesthetic benzocaine and intubation to maintain ventilation and oxygenation through the gills. The same app...

BACKGROUND Genetic studies of cardiomyopathy and heart failure have limited throughput in mammalian models. Adult zebrafish have been recently pursued as a vertebrate model with higher throughput, but genetic conservation must be tested. METHODS AND RESULTS We conducted transcriptome analysis of zebrafish heart and searched for fish homologues of 51 known human dilated cardiomyopathy-associat...

The adult human myocardium is incapable of regeneration; yet, the zebrafish (Danio rerio) can regenerate damaged myocardium. Similar to the zebrafish heart, hearts of neonatal, but not adult mice are capable of myocardial regeneration. We performed a proteomics analysis of adult zebrafish hearts and compared their protein expression profile to hearts from neonatal and adult mice. Using differen...

Though the cancer research community has used mouse xenografts for decades more than zebrafish xenografts, zebrafish have much to offer: they are cheap, easy to work with, and the embryonic model is relatively easy to use in high-throughput assays. Zebrafish can be imaged live, allowing us to observe cellular and molecular processes in vivo in real time. Opponents dismiss the zebrafish model du...

Zebrafish (Danio rerio) has become an increasingly important model for in vivo and in vitro studies on host-pathogen interaction, offering scientists with optical accessibility and genetic tractability, and a vertebrate-type immunity that can be separated into innate and adaptive ones. Although it is shown in previous studies that few species of viruses can naturally infect zebrafish, the sprin...

Comparative analysis of metagenomic DNA extraction methods from gut microbiota zebrafish (Danio rerio) for downstream next-generation sequencingVenetia D#;Rose, Tina Kollannoor Johny, Sarita Bhat