The utility of peracetic acid for sterilization of serum and yeast extract additions to mycoplasma medium was studied by culturing six Mycoplasma species. Culture media containing additions that had been sterilized with peracetic acid proved to be as good as filtered components. The use of 0.05 to 0.1% peracetic acid is recommended to sterilize the serum and yeast extract additions since saving...

A. atrocyaneus ATCC 13752 was grown in yeast extract-dextrose-broth (1% peptone from casein, 0,5% yeast extract (Cenovis), 0,5% glucose, pH = 7,2) at 30 °C and harvested in the stationary phase. Prior to harvesting the cell suspension was kept at 100 °C for 30 min to inactivate autolytic enzymes. Cell walls were prepared by the usual technique 5. Paper chromatogra­ phy was carried out on Schlei...

We compared growth characteristics of 46 Legionella strains grown on buffered charcoal yeast extract alpha (BCYE alpha) agar and buffered starch yeast extract (BSYE) agar and MICs of macrolides, azalides, and fluoroquinolones for these organisms. Growth was poor and not reproducible on BSYE agar. Growth was excellent on BCYE alpha, and MICs were easy to interpret. BCYE alpha is superior to BSYE...

Resuscitation rates of injured Listeria monocytogenes on conventional selective Listeria enrichment broth and nonselective Trypticase soy broth containing 0.6% yeast extract were compared. Cells were heated to 60 degrees C for 5 min or frozen at -20 degrees C for 7 days. Inoculation of Trypticase soy broth-yeast extract with the stressed cells resulted in growth that was superior to that in Lis...

mgm. per cent 0 0 0 0 0 0 ++++ Filtered yeast extract (1/100)............ ++++ ++++ ++++ ++++ ++++ ++++ Autoclaved yeast extract (1/100) .0 0 0 0 0 ++++ Glutamine (filtered) ..001 0 0 0 0 0 ++++ Glutamine (filtered) ....... .01 0t 4 O ++++ Glutamine (filtered)....... .1 ++ ++ ++ + + ++++ Glutamine (filtered)....... 1 ++++++++++++ ++++++++++ Glutamine (filtered)....... 10 ++++ ++++ ++++ ++++++++...

Several isolates of Gram-positive, acidophilic, moderately thermophilic, ferrous-iron- and mineral-sulphide-oxidizing bacteria were examined to establish unequivocally the characteristics of Sulfobacillus-like bacteria. Two species were evident: Sulfobacillus thermosulfidooxidans with 48-50 mol% G+C and Sulfobacillus acidophilus sp. nov. with 55-57 mol% G+C. Both species grew autotrophically an...

single male fly was housed in inner vial, with water in outer vial, to keep a high humidity inside. Two kinds of liquid food were provided to the fly through two capillaries separately. One contains 5% sucrose and 5% yeast extract, which is represented by green color. The other contains 5% sucrose, 5% yeast extract and ethanol in a certain concentration, which is represented by red color. The t...

Three new records of Ascomycota species (Chaetomium acropullum, Phialemonium globosum, Phialemonium atrogriseum) from field soils in Korea are presented in this study. These newly discovered fungal isolates were isolated from field soils from various places across Gyeongnam, Korea in 2016. All the isolates were identified and described based on morphological characteristics, and rDNA internal t...

Exopolysaccharides (EPS) of fungal origin have attracted special attention from researchers due to their multifarious applications in the food and pharmaceutical industries. In the present study, optimization of the process parameters for the production of exopolysaccharide by Schizophyllum commune AGMJ-1 was studied using one factor at a time (OFAT) method, Plackett-Burman design (PBD) and res...

Freshly isolated clinical strains of Klebsiella were tested for the ability to fix dinitrogen by the acetylene reduction assay. Ability to detect this trait was markedly affected by cultural conditions. When the test was run at 37 degrees C in the presence of yeast extract (50 mg/liter), only 1.6% of the organisms were diazotrophs, whereas this temperature without yeast extract yielded 12.9% po...