In this research, the effects of zeatin riboside (0.6, 0.8, 1, 1.2 and 1.4 mg l-1), duration of heat stress of 35 °C (6, 8, 10 and 12 days) and mannitol (0, 10, 20, 30 and 40 mg l-1) on anther culture of four eggplant cultivars were investigated in independent experiments. These experiments were performed according to a factorial arrangement in a randomized complete blocks design layout. The re...

the latest micromanipulative technique, intracytoplasmic sperm injection (icsi), has been very successful in cases of severe male factor infertility. the main objective of this study is to report the results of our first experience with icsi in cases of male factor infertility, as well as patients who had failed their previous cycles with conventional in vitro fertilization (ivf). normal fertil...

Pollen tubes must navigate through different female tissues to deliver sperm to the embryo sac for fertilization. Protein disulfide isomerases play important roles in the maturation of secreted or plasma membrane proteins. Here, we show that certain T-DNA insertions in Arabidopsis thaliana PDIL2-1, a protein disulfide isomerase (PDI), have reduced seed set, due to delays in embryo sac maturatio...

In micromanipulation experiments using immature oocytes, final ooplasmic maturation is often compromised because the oocytes are usually first freed from their nurturing cumulus cells. This study was undertaken to determine whether cumulus-free in vitro maturation (IVM) in mice could be improved by modifying IVM medium having defined components. Cumulus-free germinal vesicle (GV) stage oocytes ...

The past several decades have seen tremendous advances in the field of medical genetics. The application of genetic technologies to the field of reproductive medicine has ushered in a new era of medicine that is likely to greatly expand in the coming years. Concurrent with an in vitro fertilization (IVF) cycle, it is now possible to obtain a cellular biopsy from a developing embryo and genetica...

Phosphoinositides play important roles in plant growth and development. Several SAC domain phosphoinositide phosphatases have been reported to be for Here, we show functional analysis of SUPPRESSOR OF ACTIN 6 (SAC6) SAC8 Arabidopsis, a subfamily containing SAC-domain two transmembrane motifs. We isolated an Arabidopsis mutant ncp2 that lacked cotyledons seedling embryo pid, background defective...

BACKGROUND Frozen embryo transfers are characterized by impaired pregnancy outcome and increased incidence of pregnancy loss as compared with fresh IVF/ICSI embryo transfers. In this study, we performed a retrospective analysis of clinical and embryological factors that potentially influence the outcome of frozen embryo transfer. METHODS We reviewed the outcome of 1242 frozen embryo transfers...

The extent to which the morphology of the oocyte at the light microscopy level is related to the results of intracytoplasmic sperm injection (ICSI) is controversial. In this study, after cumulus removal, oocytes were graded into four groups according to the status of the first polar body, size of the perivitelline space and the presence of cytoplasmic inclusions. Oocyte data from 65 consecutive...