BACKGROUND Xylose reductase (XR) and xylitol dehydrogenase (XDH) from Pichia stipitis are the two enzymes most commonly used in recombinant Saccharomyces cerevisiae strains engineered for xylose utilization. The availability of NAD+ for XDH is limited during anaerobic xylose fermentation because of the preference of XR for NADPH. This in turn results in xylitol formation and reduced ethanol yie...

The effect of the growth rate and oxygen transfer rate on Debaryomyces hansenii physiology was studied using xylose-limited and oxygen-limited chemostat cultures respectively, and complemented with enzymatic assays. Under xylose-limited chemostat (oxygen-excess) neither ethanol nor xylitol was produced over the entire range of dilution rate (D). The maximal volumetric biomass productivity was 2...

Background: Reactive Oxygen Species (ROS) play a critical role in development of number pathologies. Xanthine Oxidase (XO) as well as the Xanthine Dehydrogenase (XDH) are two enzymes responsible for the last steps of purine catabolism, hydroxylation of a wide variety pyrimidines, and the initiation of ROS synthesis. In our current experiments we have analyzed whether exogenously added allopurin...

Centella asiatica is a traditional natural medicine used in number of Southeast Asian nations. (also known as L., Urb., or Gotu Kola). The aim this study to determine the antihyperuricemia properties extract and possibility that they will interact with XDH enzyme. enzyme one three enzymes can influence onset hyperuricemia other two are SLC22A12 ABCG2. In research, we employ computational method...

Pichia stipitis CBS 6054 will grow on D-xylose, D-arabinose, and L-arabinose. D-Xylose and L-arabinose are abundant in seed hulls of maize, and their utilization is important in processing grain residues. To elucidate the degradation pathway for L-arabinose, we obtained a mutant, FPL-MY30, that was unable to grow on D-xylose and L-arabinose but that could grow on D-arabinitol. Activity assays o...

Electrophoretic studies of isozymes were done with four closely related species of the Simulium perflavum species group (Diptera: Simuliidade) in the Brazilian Amazon, using last-instar larvae collected in the field. Ten enzymes were studied, which yielded 11 loci. Diagnostic loci were not found between Simulium maroniense cytotype D and Simulium rorotaense. Simulium maroniense and S. rorotaens...

background:  the development of nanotechnology has been a boon to mankind as its significance paved the way for several applications in therapeutics. this study was to evaluate the anti-arthritic capability of nano selenium-lovastatin mixture (lov-se) against inflammatory cascade in arthritic irradiated rats. materials and methods: animal model of arthritis was organized by subcutaneous injecti...