Lead is a potent environmental toxin that has accumulated above its natural level as a result of human activity. Pb cation shows major affinity towards protein complexation and it has been used as modulator of protein-membrane interactions. We located the binding sites of Pb(II) with human serum (HSA) and bovine serum albumins (BSA) at physiological conditions, using constant protein concentrat...

The interaction of crythrosine B (ErB), a commonly used dye for coloring foods and drinks, with bovine scrum albumin (BSA) was investigated both in the absence and presence of bilirubin (BR) using absorption and absorption difference spectroscopy. ErB binding to BSA was reflected from a significant red shift of 11 nm in the absorption maximum of ErB (527 nm) with the change in absorbance at lam...

the aim of this study was to evaluate the effect of bovine serum albomin (bsa) in stallion semen extender in cool condition. after collection, semen samples were pooled and divided into four equal parts. each part was diluted with one of the following extenders: 1) extender without bsa (bsa-0), 2) extender containing 5 mg/ml bsa (bsa-5), 3) extender containing 10 mg/ml bsa (bsa-10), and 4) exte...

The effect of bovine serum albumin (BSA) on drug absorption from the liver surface in rats was examined by using three organic anions (phenol red, bromphenol blue and bromosulphonphthalein) as model drugs which have a high affinity for albumin. The binding ratio of the model drugs (3 mg/ml in phosphate buffer) to BSA varied widely at a BSA concentration of 0.1--10% (w/v). The model drugs (3 mg/...

The interaction of bllirubin at concentrations less than 25 PM with bovine serum albumin (BSA) in aqueous solutions ranging from pH 3.5 to 8.6 was studied at room temperature by using the methods of optical rotatory dispersion (ORD) and absorption spectrophotometry between 230 and 600 mp. The binding of bilirubin by BSA at pH 5 caused a red shift of an absorption maximum from about 440 to 457 r...

The primary structure of catfish (Silurus asotus) egg lectin (SAL) was determined. SAL cDNA contained 1448-bp nucleotides and 308 amino acid residues, deduced from open reading frame. The SAL mature protein composed of 285-amino acid residues was followed by a predicted signal sequence having 23 residues. The mRNA of SAL was found to be expressed in eggs, but not in liver. SAL is composed of th...

Vanillin is a plant secondary metabolite and has numerous beneficial health applications. Divanillin is the homodimer of vanillin and used as a taste enhancer compound and also a promissory anticancer drug. Here, divanillin was synthesized and studied in the context of its interaction with bovine serum albumin (BSA). We found that divanillin acquires axial chirality when complexed with BSA. Thi...